Testing Data
(Published customer image: Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 um. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5 -6/group, ** p)
Mouse MHC CLASS II H-2I-Ak/s Monoclonal Antibody
MOUSE ANTI MHC CLASS II H-2I-Ak/s
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results
Preparation
Fusion Partners
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image: Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 um. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5 -6/group, ** p)
Testing Data
(Published customer image: Progressive degeneration of the nigral dopaminergic neurons after intrastriatal LPS. (A) Representative TH immunostaining of coronal midbrain sections demonstrates that the numbers of TH-positive neurons and fibers in the substantia nigra pars compacta are gradually reduced by intrastriatal LPS injection. Note that TH-positive neurons in the medial substantia nigra pars compacta and ventral tegmental area are spared; scale bar: 200 um. (B) Stereological cell counts of the TH-positive neurons in the substantia nigra pars compacta (n = 5 -6/group, ** p)
Testing Data
(Published customer imageEffect of levodopa/benserazide treatment on MHC II+cells in the corpus callosum (CC) after tMCAO. (A) Number of MHC II+ cells in the CC contralateral to the lesioned hemisphere of rats treated with vehicle (n?=?7) or levodopa/benserazide (n?=?6). Data are presented as means?+/-?SEM and statistical difference was tested with the Student's t-test, P?)
Testing Data
(Published customer imageEffect of levodopa/benserazide treatment on MHC II+cells in the corpus callosum (CC) after tMCAO. (A) Number of MHC II+ cells in the CC contralateral to the lesioned hemisphere of rats treated with vehicle (n?=?7) or levodopa/benserazide (n?=?6). Data are presented as means?+/-?SEM and statistical difference was tested with the Student's t-test, P?)
Testing Data
(Published customer imageReduction of MHC II protein and pro-inflammatory cytokines 14 days after tMCAO. (A) Co-localization of MHC II (Cy3, red) and D1 receptors (Alexa 488, green) in the peri-infarct area 14 days after tMCAO; two cells are indicated with an arrow. Higher magnification of the area delineated in the merged low power micrograph. The asterisk indicates the infarct core. Scale bars: low magnification - 50 um; high magnification - 5 um. (B) Western blot analysis for MHC II in the infarct core of rats treated either with vehicle (VH, n?=?4) or levodopa/benserazide (LD, n?=?4). Levels of MHC II protein are presented as arbitrary units (AU) to the right. Data are displayed as means?+/-?SEM, Student's t-test, P?)
Testing Data
(Published customer imageReduction of MHC II protein and pro-inflammatory cytokines 14 days after tMCAO. (A) Co-localization of MHC II (Cy3, red) and D1 receptors (Alexa 488, green) in the peri-infarct area 14 days after tMCAO; two cells are indicated with an arrow. Higher magnification of the area delineated in the merged low power micrograph. The asterisk indicates the infarct core. Scale bars: low magnification - 50 um; high magnification - 5 um. (B) Western blot analysis for MHC II in the infarct core of rats treated either with vehicle (VH, n?=?4) or levodopa/benserazide (LD, n?=?4). Levels of MHC II protein are presented as arbitrary units (AU) to the right. Data are displayed as means?+/-?SEM, Student's t-test, P?)
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MBS214435))
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: FITC (MBS214435))
Testing Data
(Published customer image: Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic disease. A, low power view of midbrain reveals enhanced lectin staining in the red nucleus. B, higher magnification shows that enhanced lectin reactivity is confined strictly to red nucleus region (arrows indicate perimeter of red nucleus). C, microglial fusions are interspersed with rubrospinal neurons that appear undamaged. D, lectin-positive microglial fusion (giant cell) within red nucleus. E, oculomotor nucleus reveals normal-appearing motor neurons and lack of microgliosis. F, substantia nigra (pars compacta) shows presence of normal, ramified microglial cells. G, motor cortex shows normal, ramified microglia. H, single, ramified microglial cell positive with OX-6 (arrow) near lateral ventricle. Scale bars: 400 um (A); 200 um (E); 100 um (B,H); 50 um (C,F,G); 20 um (D).From: Fendrick SE, Xue QS, Streit WJ. Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene. J Neuroinflammation. 2007 Feb 28;4:9.)
Testing Data
(Published customer image: Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during symptomatic disease. A, low power view of midbrain reveals enhanced lectin staining in the red nucleus. B, higher magnification shows that enhanced lectin reactivity is confined strictly to red nucleus region (arrows indicate perimeter of red nucleus). C, microglial fusions are interspersed with rubrospinal neurons that appear undamaged. D, lectin-positive microglial fusion (giant cell) within red nucleus. E, oculomotor nucleus reveals normal-appearing motor neurons and lack of microgliosis. F, substantia nigra (pars compacta) shows presence of normal, ramified microglial cells. G, motor cortex shows normal, ramified microglia. H, single, ramified microglial cell positive with OX-6 (arrow) near lateral ventricle. Scale bars: 400 um (A); 200 um (E); 100 um (B,H); 50 um (C,F,G); 20 um (D).From: Fendrick SE, Xue QS, Streit WJ. Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene. J Neuroinflammation. 2007 Feb 28;4:9.)
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor 488 )
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor 488 )
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s (MBS214438))
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s (MBS214438))
Testing Data
(Published customer image: Activation of microglial cells. Vertical section of retinas from a SD (A-C), untreated P23H (D-F) and tauroursodeoxycholic acid (TUDCA)-treated P23H (G-I) rat stained for Iba1 (green; A, D, G) MHC-II RT 1B (red; B, E, H) or both (C, F, I). Nuclei stained with a nuclear marker (TO-PRO 3, blue). All images were collected from the central area of the retina, close to the optic nerve. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar: 20 um.From: Noailles A, Fern¡ndez-S¡nchez L, Lax P, Cuenca N. Microglia activation in a model of retinal degeneration and TUDCA neuroprotective effects. J Neuroinflammation. 2014 Oct 29;11(1):186.)
Testing Data
(Published customer image: Activation of microglial cells. Vertical section of retinas from a SD (A-C), untreated P23H (D-F) and tauroursodeoxycholic acid (TUDCA)-treated P23H (G-I) rat stained for Iba1 (green; A, D, G) MHC-II RT 1B (red; B, E, H) or both (C, F, I). Nuclei stained with a nuclear marker (TO-PRO 3, blue). All images were collected from the central area of the retina, close to the optic nerve. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar: 20 um.From: Noailles A, Fern¡ndez-S¡nchez L, Lax P, Cuenca N. Microglia activation in a model of retinal degeneration and TUDCA neuroprotective effects. J Neuroinflammation. 2014 Oct 29;11(1):186.)
Testing Data
(Published cusromer image: OX-6 IHC staining in the DG. No OX-6 positive cells were identified in P1 D1D2 pups treated with either NS (a) or Dex (b). Brain tumor implant staining as a positive control showed OX-6 positive cells stained brown (c), arrow). Magnification, 400x; scale bar, 50?um.From: Sze CI, Lin YC, Lin YJ, Hsieh TH, Kuo YM, Lin CH. The role of glucocorticoid receptors in dexamethasone-induced apoptosis of neuroprogenitor cells in the hippocampus of rat pups. Mediators Inflamm. 2013;2013:628094.)
Testing Data
(Published cusromer image: OX-6 IHC staining in the DG. No OX-6 positive cells were identified in P1 D1D2 pups treated with either NS (a) or Dex (b). Brain tumor implant staining as a positive control showed OX-6 positive cells stained brown (c), arrow). Magnification, 400x; scale bar, 50?um.From: Sze CI, Lin YC, Lin YJ, Hsieh TH, Kuo YM, Lin CH. The role of glucocorticoid receptors in dexamethasone-induced apoptosis of neuroprogenitor cells in the hippocampus of rat pups. Mediators Inflamm. 2013;2013:628094.)
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor 647 )
Testing Data
(Staining of rat spleen cells with Mouse anti H-2I-Ak/s: Alexa Fluor 647 )
Similar Products
Product Notes
The MHC CLASS II H-2I-Ak/s (Catalog #AAA214441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II H-2I-Ak/s can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*, Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100 Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results. Researchers should empirically determine the suitability of the MHC CLASS II H-2I-Ak/s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II H-2I-Ak/s, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
