Human low density lipoprotein receptor ELISA Kit | LDLR elisa kit

Human low density lipoprotein receptor, LDLR ELISA Kit

Cat. Num. AAA700082

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Human
• Synonyms: low density lipoprotein receptor; Human low density lipoprotein receptor; LDLR ELISA Kit; FH; FHC; LDLCQ2; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; LDLR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LDLR. No significant cross-reactivity or interference between human LDLR and analogues was observed.
• Sequence Length: 860

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 47 pg/ml -3000 pg/ml.
• Sensitivity: The minimum detectable dose of human LDLR is typically less than 11.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDLR elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDLR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDLR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504975
• NCBI GeneID: 3949
• NCBI Accession #: NP_000518.1
• NCBI GenBank Nucleotide #: NM_000527.4
• UniProt Accession #: P01130; Q53ZD9; Q59FQ1; Q9UDH7; B4DII3; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; H0YLU8; H0YNT7
• Molecular Weight: 95,376 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

Function: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.