Typical Testing Data/Standard Curve (for reference only)
Pig insulin-like growth factors 2 ELISA Kit | IGF-2 elisa kit
Pig insulin-like growth factors 2 (IGF-2) ELISA Kit
Gene Names
IGF2; IGF-II
Reactivity
Pig
Synonyms
insulin-like growth factors 2; Pig insulin-like growth factors 2 (IGF-2) ELISA Kit; IGF-2 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig IGF2. No significant cross-reactivity or interference between Pig IGF2 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IGF-2 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IGF2 in samples. An antibody specific for IGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,313 Da
NCBI Official Full Name
insulin-like growth factor II preproprotein
NCBI Official Symbol
IGF2
NCBI Official Synonym Symbols
IGF-II
NCBI Protein Information
insulin-like growth factor II
UniProt Protein Name
Insulin-like growth factor II
UniProt Gene Name
IGF2
UniProt Synonym Gene Names
IGF-II
Uniprot Description
The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver.
Research Articles on IGF-2
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Product Notes
The Pig IGF-2 igf2 (Catalog #AAA282320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282320 ELISA Kit recognizes Pig IGF-2. It is sometimes possible for the material contained within the vial of "insulin-like growth factors 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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