Mouse MHC CLASS I H-2Kb Monoclonal Antibody | anti-H2-K1 antibody

MOUSE ANTI MOUSE MHC CLASS I H-2Kb

Cat. Num. AAA219674

• Gene Names: H2-K1; K-f; H-2K; H2-K; H-2K(d)
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: MHC CLASS I H-2Kb; Monoclonal Antibody; MOUSE ANTI MOUSE MHC CLASS I H-2Kb; anti-H2-K1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: AF6-88.5
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 369

• Applicable Applications for anti-H2-K1 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: C57BL mouse splenocytes; Buffer Solution
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-H2-K1 antibody

Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene. Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.

NCBI and Uniprot Product Information

• NCBI GI #: 133922588
• NCBI GeneID: 14972
• NCBI Accession #: NP_001001892.2
• NCBI GenBank Nucleotide #: NM_001001892.2
• UniProt Accession #: P01901; P01902; P03991; P04223; P14428
• Molecular Weight: 41,302 Da
• NCBI Official Full Name: H-2 class I histocompatibility antigen, K-W28 alpha chain isoform 1
• NCBI Official Synonym Full Names: histocompatibility 2, K1, K region
• NCBI Official Symbol: H2-K1
• NCBI Official Synonym Symbols: K-f; H-2K; H2-K; H-2K(d)
• NCBI Protein Information: H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC H2-K transplantation antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I antigen; MHC class I heavy chain H2-K; class I major histocompatibility antigen H-2Kd
• UniProt Protein Name: H-2 class I histocompatibility antigen, K-B alpha chain
• Protein Family: H-2 class I histocompatibility antigen
• UniProt Gene Name: H2-K1
• UniProt Synonym Gene Names: H2-K; H-2K(B)
• UniProt Entry Name: HA1B_MOUSE

Uniprot Description

HLAC iso22: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: Golgi apparatus; cell surface; membrane; endoplasmic reticulum; integral to membrane; MHC class I protein complex; Golgi medial cisterna; external side of plasma membrane

Molecular Function: protein binding; peptide antigen binding; protein heterodimerization activity; beta-2-microglobulin binding; T cell receptor binding; peptide binding; TAP binding; receptor binding

Biological Process: antigen processing and presentation; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; immune system process; defense response to bacterium; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; inner ear development; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent