Testing Data
(Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC (MBS216456))
Mouse MHC CLASS I RT1A Monoclonal Antibody
MOUSE ANTI RAT MHC CLASS I RT1A
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
MHC CLASS I RT1A; Monoclonal Antibody; MOUSE ANTI RAT MHC CLASS I RT1A; anti-MHC CLASS I RT1A antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-18
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-MHC CLASS I RT1A antibody
Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Rat spleen glycoproteins
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC (MBS216456))
Testing Data
(Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC (MBS216456))
Related Product Information for anti-MHC CLASS I RT1A antibody
Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (Fukumoto et al. 1982). Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on rat splenocytes.
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Product Notes
The MHC CLASS I RT1A (Catalog #AAA213068) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS I RT1A can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the MHC CLASS I RT1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS I RT1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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