Mouse anti-Human MHC Class I Monoclonal Antibody

anti-human MHC Class I-Biotin

Cat. Num. AAA666614

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: MHC Class I; Monoclonal Antibody; anti-human MHC Class I-Biotin; Monoclonal anti-human CD326(EpCAM)/Biotin; anti-MHC Class I antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: ANC8D4
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 354

• Applicable Applications for anti-MHC Class I antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Performance: Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD326/Biotin at concentration of 5 ug/ml. They were then washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with unlabeled anti-human CD326 mAb.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant Human CD326
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-MHC Class I antibody

Information: Human CD326 (EpCAM, EGP2, HEA) is a 40kD glycoprotein expressed on epithelial cells and many carcinomas. It is an adhesion molecule whose function is implicated in carcinogenesis and erythropoesis. Antibody ANC8D4 recognizes recombinant CD326 in EIA, and cell surface human CD326 in FACS.

References

Rao CG, et al.. (2005) Int. J. Oncol. 27: 49.

NCBI and Uniprot Product Information

• NCBI GI #: 1545844
• Molecular Weight: 39,699 Da
• NCBI Official Full Name: MHC class I, partial
• UniProt Protein Name: MHC class I
• Protein Family: MHC class II transactivator
• UniProt Entry Name: Q95430_PONPY

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.