Testing Data
Mouse anti-Human LTbetaR Antibody | anti-Ltbr antibody
anti-human LTbetaR-Biotin
Gene Names
Ltbr; CD18; Ltar; TNFCR; Tnfbr; TNFRrp; LTbetaR; TNFR-RP; Tnfrsf3; AI256028; TNFR2-RP; TNF-R-III
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
LTbetaR; anti-human LTbetaR-Biotin; anti-human LTbR (Lymphotoxin beta receptor)/Biotin; anti-Ltbr antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1k
Clone Number
ANCLTR2/9E2
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
415
Applicable Applications for anti-Ltbr antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Performance
Five x 105 cultured human U-937 cells were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-LTbR/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.64 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre blocked with 20ul of 0.5 mg/ml unlabeled anti-LTbR antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Recombinant Human LT
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-Ltbr antibody
Information: Human Lymphotoxin beta receptor (LTbetaR, TNFRSF3) is a member of the TNF receptor superfamily with similarilty to CD120a(TNFR1) and CD120b(TNFR2). It is a expressed mainly on non lymphoid tissues(1) and appears to have an important role in secondary lymphoid organ development(5). Suppression of LTbetaR signaling can alleviate autoimmunity(2) or exasperate mycobacterial infection(6). Its ligands include Ltab2 and LIGHT. In mice, LTbetaR signaling is important for development and function of HEV(2), Dendritic cells(3), and Mast cells(4).
Antibody ANCLTR2 binds to human LTbetaR present on U-937 cell surface, and to recombinant LTbetaR-muIg in EIA.
References
1) T W Mak, M E Saunders eds. The Immune Response 2006 Elsevier Academic Press p 499.
2) J L Browning, R.A. Fava, et al. Immunity (2005) 23(5): 539-50. 3)Y Wang, Y Fu, et al. J Immunol (2005) 175: 6997-7002. 4) P Stopfer, T Hehlgans, et al. J Immunol (2004) 172: 7459-7465. 5) Gommermann J, JL Browning, et al, Nat Rev Immunol (2003) 3:642-655. 6) Spahn TW, T Kucharzik, et al. Infection and Immunity (2005) 73(11): 7077-7088.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,956 Da
NCBI Official Full Name
Lymphotoxin B receptor
NCBI Official Synonym Full Names
lymphotoxin B receptor
NCBI Official Symbol
Ltbr
NCBI Official Synonym Symbols
CD18; Ltar; TNFCR; Tnfbr; TNFRrp; LTbetaR; TNFR-RP; Tnfrsf3; AI256028; TNFR2-RP; TNF-R-III
NCBI Protein Information
tumor necrosis factor receptor superfamily member 3; LT beta-R; LT-beta receptor; lymphotoxin-beta receptor; TNF receptor-related protein; tumor necrosis factor receptor superfamily, member 3
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 3
Protein Family
UniProt Gene Name
Ltbr
UniProt Synonym Gene Names
Tnfcr; Tnfrsf3
UniProt Entry Name
TNR3_MOUSE
Uniprot Description
LTBR: Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane
Molecular Function: identical protein binding; protein binding; ubiquitin protein ligase binding
Biological Process: apoptosis; positive regulation of JNK cascade; myeloid dendritic cell differentiation; lymph node development
Research Articles on Ltbr
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Product Notes
The Ltbr ltbr (Catalog #AAA666776) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human LTbetaR-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LTbetaR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the Ltbr ltbr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LTbetaR, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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