Testing Data
Mouse anti-Human MHC Class I Monoclonal Antibody
anti-human MHC Class I-Purified Preservative Free
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Preservative Free
Synonyms
MHC Class I; Monoclonal Antibody; anti-human MHC Class I-Purified Preservative Free; Monoclonal anti-human MHC Class I (HLA-A; B and C); anti-MHC Class I antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3F10
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Sequence Length
354
Applicable Applications for anti-MHC Class I antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)
Performance
Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-MHC Class I antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG2a isotype control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human HSB-2 T cell line
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-MHC Class I antibody
Information: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane alpha chain and an invariant beta chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules. Antibody 3F10 recognizes a monomorphic Class I epitope on the a chain.
References
G.S. Eisenbarth, et al, (1980) J Immunol 124: 1237-1244. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194. K.L. Rock, (1996) Immunol Today 17: 131-137.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
39,699 Da
NCBI Official Full Name
MHC class I, partial
UniProt Protein Name
MHC class I
Protein Family
UniProt Entry Name
Q95430_PONPY
Uniprot Description
Involved in the presentation of foreign antigens to the immune system.
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Product Notes
The MHC Class I (Catalog #AAA666513) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human MHC Class I-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA). Researchers should empirically determine the suitability of the MHC Class I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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