Mouse MHC Class 1, H2, Db, alpha3 Monoclonal Antibody

MHC Class 1, H2, Db, alpha3

Cat. Num. AAA602196

• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Purity: Purified
• Synonyms: MHC Class 1; H2; Db; alpha3; Monoclonal Antibody; Anti -MHC Class 1; anti-MHC Class 1; alpha3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 28-14-8
• Specificity: Mouse H-2D^b
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.

• Applicable Applications for anti-MHC Class 1, H2, Db, alpha3 antibody: Flow Cytometry - Quality tested; Immunohistochemistry - Reported in literature; Immunoprecipitation - Reported in literature; Blocking - Reported in literature; Complement Mediated Cell Depletion - Reported in literature
• Application Notes: Flow Cytometry; Purified (UNLB) antibody - <= 1 ug/10⁶ cells; FITC, BIOT, and AF488 conjugates - <=1 ug/10⁶ cells; PE conjugate - <= 0.5 ug/10⁶ cells; APC conjugate - <= 0.1 ug/10⁶ cells; ; For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL.; ; Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
• Immunogen: C3H.SW mouse splenocytes
• Preparation and Storage: Store at 2-8°C.

Testing Data

Testing Data

(BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Db-UNLB (MBS602196) followed by Goat Anti-Mouse IgG2a, Human ads-FITC (MBS674471).)

Related Product Information for anti-MHC Class 1, H2, Db, alpha3 antibody

The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.

Product Categories/Family for anti-MHC Class 1, H2, Db, alpha3 antibody

Antibodies; Abs to MHC