Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Ribonuclease A3 Polyclonal Antibody | anti-RNASE3 antibody
Polyclonal Antibody to Ribonuclease A3 (RNASE3)
Gene Names
Ear11; R1; R7; EAR; ECP; Rnase3
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Ribonuclease A3; Polyclonal Antibody; Polyclonal Antibody to Ribonuclease A3 (RNASE3); anti-RNASE3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RNASE3. It has beenselected for its ability to recognize RNASE3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WFEIQHIY NRAYPRCNDA MRHRNRFTGH CKDINTFLHT SFASVVGVCG NRNIPCGNRT YRNCHNSRYR VSITFCNLTT
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WFEIQHIY NRAYPRCNDA MRHRNRFTGH CKDINTFLHT SFASVVGVCG NRNIPCGNRT YRNCHNSRYR VSITFCNLTT
Applicable Applications for anti-RNASE3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
RNASE3 (Trp33~Phe155)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant RNASE3 (Trp33~Phe155) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058725
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Skeletal muscle Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Skeletal muscle Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,007 Da
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
eosinophil-associated, ribonuclease A family, member 11
NCBI Official Symbol
Ear11
NCBI Official Synonym Symbols
R1; R7; EAR; ECP; Rnase3
NCBI Protein Information
eosinophil cationic protein
UniProt Protein Name
Eosinophil cationic protein
Protein Family
UniProt Gene Name
Rnase3
UniProt Synonym Gene Names
Ear11; Rns3; ECP; EAR-11; RNase 3
NCBI Description
cationic protein with similarity to human ECP; also has similarity to human eosinophil-derived neurotoxin and human ribonuclease k6 [RGD, Feb 2006]
Uniprot Description
Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities ().
Research Articles on RNASE3
Similar Products
Product Notes
The RNASE3 rnase3 (Catalog #AAA2028516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Ribonuclease A3 (RNASE3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the RNASE3 rnase3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WFEIQHIY NRAYPRCNDA MRHRNRFTGH CKDINTFLHT SFASVVGVCG NRNIPCGNRT YRNCHNSRYR VSITFCNLTT. It is sometimes possible for the material contained within the vial of "Ribonuclease A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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