Mouse anti-Human MGMT Monoclonal Antibody | anti-Mgmt antibody

MGMT (O6-Methylguanine-DNA Methyltransferase)

Cat. Num. AAA602861

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Ascites; Ascities
• Synonyms: MGMT; Monoclonal Antibody; MGMT (O6-Methylguanine-DNA Methyltransferase); Anti -MGMT (O6-Methylguanine-DNA Methyltransferase); anti-Mgmt antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3C157
• Specificity: Specific for MGMT (ref 1).
• Purity/Purification: Ascites; Ascities
• Form/Format: Supplied as ascites, 0.1% sodium azide.

• Applicable Applications for anti-Mgmt antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Dilution: Western Blot: 1:1,000~1:3,000 (ECL); Immunohistochemistry: 1:2,000 (strong [+++] with CEM and no signal [-] with TK6)
• Immunogen: Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli
• Positive Control: LS174T, Raji, CCRF-CEM,1 Molt-4,1 HeLa CCL2,1 Rh18,1 or HT-292 cells. Liver tissue expresses high levels while brain and bone marrow have low levels of MGMT protein. Use TK6 cells as a negative control.
• Preparation and Storage: -20 degree C

Related Product Information for anti-Mgmt antibody

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. MGMT activity varies among groups of tumors and within a particular type of tumor.

Cancer chemotherapeutic alkylating agents (e.g. BCNU, CCNU, DTIC, procarbazine, temozolomide), act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. Because MGMT repairs the potentially cytotoxic, antitumor lesions induced in DNA by alkylating agents, tumors with low or no levels of MGMT will predictably be responsive to such drug therapy. Conversely, tumors with high levels of MGMT will likely be drug resistant. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Abnormally low levels of MGMT in normal individuals/tissues may be an early marker of susceptibility to cancer.

Product Categories/Family for anti-Mgmt antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 127071
• NCBI GeneID: 25332
• UniProt Accession #: P24528
• Molecular Weight: 22,244 Da
• NCBI Official Full Name: Methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: Mgmt
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase; 0-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: Mgmt
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_RAT

NCBI Description

enzyme involved in DNA repair of O(6)-alkylguanine which is the major mutagenic and carcinogenic lesion in DNA [RGD, Feb 2006]

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Nuclear receptor co-regulator; Apoptosis; Methyltransferase; DNA repair, damage; EC 2.1.1.63

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding

Biological Process: response to drug; methylation; positive regulation of DNA repair; DNA dealkylation; response to ethanol; response to folic acid; response to toxin; regulation of caspase activity; DNA repair; response to organic cyclic substance; response to DNA damage stimulus