Mouse MEK1 Monoclonal Antibody | anti-MEK1 antibody

MEK1 antibody

• Gene Names: MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• Applications: ELISA, Western Blot
• Purity: MEK1 antibody was purified by ammonium sulfate precipitation
• Synonyms: MEK1; Monoclonal Antibody; MEK1 antibody; Monoclonal MEK1; Anti-MEK1; MAP2K1; mitogen-activated protein kinase kinase 1; BOS_10196; CH73-94F11.4; dual specificity mitogen activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 1; EGK_17610; ERK activator kinase 1; map2k2; MAPK/ERK kinase 1; MAP kinase/Erk kinase 1; MAP kinase kinase 1; MAP kinase kinase or Erk Kinase; Dual specificity mitogen-activated protein kinase kinase; involved in ras mediated vulval induction; LEThal LET-537 (42.8 kD) (mek-2); mapkk1; MAPKK 1; MEK; mek1; MEK 1; mek-2; MEKK1; mitogen activated protein kinase kinase 1; mkk1; PANDA_002194; PRKMK1; protein kinase; mitogen-activated; kinase 1 (MAP kinase kinase 1); mitogen activated; kinase 1; p45; RCJMB04_27f19; anti-MEK1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 32G3
• Purity/Purification: MEK1 antibody was purified by ammonium sulfate precipitation
• Form/Format: Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
• Sequence Length: 354

• Applicable Applications for anti-MEK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:2000
• Biological Significance: The human MEK1 encodes 393 amino acid residues and shares 99% amino acid sequence identity with the murine MEKl and 80% with human MEK2 (400 amino acids). The MEKs constitute an evolutionarily conserved group of three highly homologous mammalian isoforms. These are the 45 kDa MEK1, its alternatively spliced form, MEK1b (43 kDa) that is thought to be inactive, and the 46 kDa MEK2. These proteins are composed of a catalytic kinase domain, which is surrounded by a regulatory N-terminal domain (80 amino acids) and a shorter C-terminal region (30 amino acids). MEKs are activated by phosphorylation of two Ser residues in their activation loop (Ser218 and Ser222 in MEK1). The activity of MEKs is regulated by additional phosphorylation/dephosphorylation processes as well.
• Cross-Reactivity: Human, Mouse, Rat
• Immunogen: MEK1 antibody was raised in Mouse using a recombinant human protein purified from E Coli(His-MEK1) as the immunogen
• Preparation and Storage: Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles

Western Blot (WB)

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Related Product Information for anti-MEK1 antibody

Mouse monoclonal MEK1 antibody

Product Categories/Family for anti-MEK1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 2196704
• NCBI GeneID: 828713
• UniProt Accession #: Q94A06; O04440; O04661
• Molecular Weight: 34,087 Da
• NCBI Official Full Name: MEK1
• NCBI Official Symbol: MEK1
• NCBI Official Synonym Symbols: ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• NCBI Protein Information: mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MKK1
• UniProt Synonym Gene Names: MEK1; AtMKK1; MAP kinase kinase 1
• UniProt Entry Name: M2K1_ARATH

NCBI Description

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

Uniprot Description

MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination.