Western Blot (WB)
Rabbit MEK1 Polyclonal Antibody | anti-MEK1 antibody
Anti-MEK1 Antibody
Gene Names
MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
Reactivity
Human. Not yet tested in other species
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
MEK1; Polyclonal Antibody; Anti-MEK1 Antibody; anti-MEK1 antibody
Host
Rabbit
Reactivity
Human. Not yet tested in other species
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
354
Applicable Applications for anti-MEK1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA titer: 1:1,000-1:5,000
Western blot analysis: 1:200-500
Immunohistochemistry: 1:100-500
Optimal dilutions/concentrations should be determined by the end user.
Western blot analysis: 1:200-500
Immunohistochemistry: 1:100-500
Optimal dilutions/concentrations should be determined by the end user.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal MEK1 antibody was raised against a 16 amino acid peptide from near the N terminal residues of human MEK1 protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
Immunohistochemistry (IHC) #2
Immunohistochemistry (IHC) #2
References
(1) Senawong,T., Phuchareon,J., Ohara,O., et al. Germline mutations of MEK in cardio-facio-cutaneous syndrome are sensitive to MEK and RAF inhibition: implications for therapeutic options. Hum. Mol. Genet. 17 (3), 419-430 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,087 Da
NCBI Official Full Name
MEK1
NCBI Official Symbol
MEK1
NCBI Official Synonym Symbols
ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
NCBI Protein Information
mitogen-activated protein kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase 1
Protein Family
UniProt Gene Name
MKK1
UniProt Entry Name
M2K1_ARATH
NCBI Description
Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.
Uniprot Description
MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination.
Research Articles on MEK1
Similar Products
Product Notes
The MEK1 mkk1 (Catalog #AAA415614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MEK1 Antibody reacts with Human. Not yet tested in other species and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA titer: 1:1,000-1:5,000 Western blot analysis: 1:200-500 Immunohistochemistry: 1:100-500 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the MEK1 mkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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