Mouse anti-Human MED9 Monoclonal Antibody | anti-MED9 antibody

MED9 (MED25, Mediator of RNA Polymerase II Transcription Subunit 9, Mediator Complex Subunit 9, FLJ10193, MGC138234) (HRP)

Cat. Num. AAA6153488

• Gene Names: MED9; MED25
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MED9; Monoclonal Antibody; MED9 (MED25; Mediator of RNA Polymerase II Transcription Subunit 9; Mediator Complex Subunit 9; FLJ10193; MGC138234) (HRP); anti-MED9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 7F1
• Specificity: Recognizes human MED9.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 146

• Applicable Applications for anti-MED9 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-146 from human MED9 (NP_060489) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQQSPAPRPQSPARAREEENYSFLPLVHNIIKCMDKDSPEVHQDLNALKSKFQEMRKLISTMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCMFEIPKE
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (43.4kD).)

Testing Data

(Detection limit for recombinant GST tagged MED9 is 0.1ng/ml as a capture antibody.)

Related Product Information for anti-MED9 antibody

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Product Categories/Family for anti-MED9 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 8922273
• NCBI GeneID: 55090
• NCBI Accession #: NP_060489
• NCBI GenBank Nucleotide #: NM_018019
• UniProt Accession #: Q9NWA0
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 9
• NCBI Official Synonym Full Names: mediator complex subunit 9
• NCBI Official Symbol: MED9
• NCBI Official Synonym Symbols: MED25
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 9
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 9
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED9
• UniProt Synonym Gene Names: MED25
• UniProt Entry Name: MED9_HUMAN

NCBI Description

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

MED9: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 9 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Srb-mediator complex

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter