Porcine Azurocidin 1 ELISA Kit | AZU1 elisa kit

Porcine Azurocidin 1 ELISA Kit

Cat. Num. AAA748826

• Gene Names: AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• Reactivity: Porcine
• Synonyms: Azurocidin 1; Porcine Azurocidin 1 ELISA Kit; AZU1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AZU1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AZU1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU1 elisa kit

Principle of the Assay: AZU1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AZU1 antibody and an AZU1-HRP conjugate. The assay sample and buffer are incubated together with AZU1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AZU1 concentration since AZU1 from samples and AZU1-HRP conjugate compete for the anti-AZU1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU1 from the sample, fewer sites are left to bind AZU1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AZU1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11342670
• NCBI GeneID: 566
• NCBI Accession #: NP_001691.1
• NCBI GenBank Nucleotide #: NM_001700.3
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin preproprotein
• NCBI Official Synonym Full Names: azurocidin 1
• NCBI Official Symbol: AZU1
• NCBI Official Synonym Symbols: AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• NCBI Protein Information: azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: CAP7_HUMAN

NCBI Description

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: azurophil granule; extracellular region

Molecular Function: heparin binding; toxin binding; serine-type endopeptidase activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of cell adhesion; microglial cell activation; proteolysis; glial cell migration; defense response to Gram-negative bacterium; macrophage chemotaxis; induction of positive chemotaxis; cellular extravasation; monocyte activation; positive regulation of phagocytosis; positive regulation of MHC class II biosynthetic process; positive regulation of fractalkine biosynthetic process; protein kinase C activation; positive regulation of interleukin-1 beta biosynthetic process; protein processing; immune response; regulation of vascular permeability; inflammatory response; negative regulation of apoptosis