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Western Blot (WB) (Western Blot detection against Immunogen (43.4kD).)

Mouse anti-Human MED9 Monoclonal Antibody | anti-MED9 antibody

MED9 (MED25, Mediator of RNA Polymerase II Transcription Subunit 9, Mediator Complex Subunit 9, FLJ10193, MGC138234)

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
MED9; Monoclonal Antibody; MED9 (MED25; Mediator of RNA Polymerase II Transcription Subunit 9; Mediator Complex Subunit 9; FLJ10193; MGC138234); Anti -MED9 (MED25; anti-MED9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
7F1
Specificity
Recognizes human MED9.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQQSPAPRPQSPARAREEENYSFLPLVHNIIKCMDKDSPEVHQDLNALKSKFQEMRKLISTMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCMFEIPKE
Applicable Applications for anti-MED9 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Full length recombinant corresponding to aa1-146 from human MED9 (NP_060489) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (43.4kD).)

Western Blot (WB) (Western Blot detection against Immunogen (43.4kD).)

Testing Data

(Detection limit for recombinant GST tagged MED9 is 0.1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged MED9 is 0.1ng/ml as a capture antibody.)
Related Product Information for anti-MED9 antibody
The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
Product Categories/Family for anti-MED9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,116 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 9
NCBI Official Synonym Full Names
mediator complex subunit 9<
NCBI Official Symbol
MED9
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 9
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 9
UniProt Gene Name
MED9
UniProt Synonym Gene Names
MED25
UniProt Entry Name
MED9_BOVIN

Uniprot Description

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

By similarity.

Subunit structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP

By similarity.

Subcellular location: Nucleus

Probable.

Sequence similarities: Belongs to the Mediator complex subunit 9 family.

Similar Products

Product Notes

The MED9 med9 (Catalog #AAA6004975) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MED9 (MED25, Mediator of RNA Polymerase II Transcription Subunit 9, Mediator Complex Subunit 9, FLJ10193, MGC138234) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the MED9 med9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNAL KSKFQEMRKL ISTMPGIHLS PEQQQQQLQS LREQVRTKNE LLQKYKSLCM FEIPKE. It is sometimes possible for the material contained within the vial of "MED9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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