Mouse anti-Human MDM4 / MDMX Monoclonal Antibody | anti-MDM4 antibody

Mouse Monoclonal [clone 2D10F4] (IgG1) to Human MDM4 / MDMX

Cat. Num. AAA246589

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Ascites
• Synonyms: MDM4 / MDMX; Monoclonal Antibody; Mouse Monoclonal [clone 2D10F4] (IgG1) to Human MDM4 / MDMX; Anti-MDM4 / MDMX Antibody (clone 2D10F4) IHC-plus; MDM4; Mdm2-like p53-binding protein; MDMX; HDMX; MRP1; p53-binding protein Mdm4; Protein Mdm4; Double minute 4 protein; MDM4-related protein 1; Protein Mdmx; Human MDM4; anti-MDM4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D10F4
• Specificity: Human MDM4
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide
• Sequence Length: 490

• Applicable Applications for anti-MDM4 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:200 - 1:1000), IHC-P (1:200), WB (1:500 - 1:2000)
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of human MDM4 expressed in E Coli.
• Immunogen: MDM4 / MDMX antibody was raised against purified recombinant fragment of human MDM4 expressed in E Coli.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MDM4 / MDMX antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-MDM4 / MDMX antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunofluorescence (IF)

(Confocal immunofluorescence of HeLa (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.)

Western Blot (WB)

(Western blot of anti-MDM4 mAb against HeLa (1), A549 (2) and A431 (3) cell lysate.)

Related Product Information for anti-MDM4 antibody

The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested.

NCBI and Uniprot Product Information

• NCBI GI #: 88702791
• NCBI GeneID: 4194
• NCBI Accession #: NP_002384.2
• NCBI GenBank Nucleotide #: NM_002393.4
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 49,541 Da
• NCBI Official Full Name: protein Mdm4 isoform 1
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4; protein Mdmx; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; double minute 4, human homolog of; p53-binding protein
• UniProt Protein Name: Protein Mdm4
• Protein Family: Protein
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX
• UniProt Entry Name: MDM4_HUMAN

NCBI Description

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding

Biological Process: negative regulation of cell proliferation; cell proliferation; protein stabilization; G0 to G1 transition; DNA damage response, signal transduction by p53 class mediator; positive regulation of cell proliferation; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of apoptosis