Western Blot (WB)
(Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).)
Mouse mCherry Monoclonal Antibody | anti-mCherry antibody
Anti-mCherry Antibody
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein G Purified Culture Supernatant
Synonyms
mCherry; Monoclonal Antibody; Anti-mCherry Antibody; anti-mCherry antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1C51
Specificity
Specific for the ~28 kDa mCherry protein
Purity/Purification
Protein G Purified Culture Supernatant
Form/Format
100 ul in PBS + 50% glycerol and 5 mM NaN3
Sequence Length
236
Applicable Applications for anti-mCherry antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IF: 1:500
IF: 1:500
Immunogen Species
Mouse
Immunogen Information
Recombinant full length protein.
Antigen
Full length recombinant protein
Quality Control Tests
Western blots performed on each lot.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).)
Western Blot (WB)
(Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).)
Immunofluorescence (IF)
(Immunofluorescence of HEK293 cells transfected with mCherry (red) and immunolabeled with mCherry antibody (green) subsequently appear yellow. Untransfected cell nuclei are stained blue.)
Immunofluorescence (IF)
(Immunofluorescence of HEK293 cells transfected with mCherry (red) and immunolabeled with mCherry antibody (green) subsequently appear yellow. Untransfected cell nuclei are stained blue.)
Related Product Information for anti-mCherry antibody
mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in study of their expression and in transgenic research. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.
References
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The mCherry (Catalog #AAA500080) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's mCherry can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000 IF: 1:500. Researchers should empirically determine the suitability of the mCherry for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mCherry, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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