Western Blot (WB)
(The recombinant mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse brain (1:500))
Mouse anti-Human, Mouse PSP Monoclonal Antibody | anti-PSP antibody
PSP antibody
Gene Names
PSPH; PSP; PSPHD
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
PSP; Monoclonal Antibody; PSP antibody; PSPH; Phosphoserine phosphatase Human; PSPase; EC 3.1.3.3; O-phosphoserine phosphohydrolase; L-3-phosphoserine phosphatase; Phosphoserine phosphatase; L 3 phosphoserine phosphatase; O phosphoserine phosphohydrolase; Phosphoserine phosphatase deficiency; included; ; anti-PSP antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
3G12
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
225
Applicable Applications for anti-PSP antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
Recommended starting dilution is 1:1,000.
Antigen Species
Human
Immunogen
Recombinant human PSP (1-225aa) purified from E. coli
Clone Note
Anti-human PSP mAb, clone 3G12, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PSP protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse brain (1:500))
Western Blot (WB)
(The recombinant mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse brain (1:500))
Related Product Information for anti-PSP antibody
Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg (2+)-dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.
Product Categories/Family for anti-PSP antibody
References
Moro-Furlani, et al., (1980) Ann Hum Genet 43 (4):323-3233; Kim HY, et al., (2002) J Biol Chem 277 (48):46651-46658; Peeraer Y, et al., (2004) Eur J Biochem 271 (16):3421-3427
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,008 Da
NCBI Official Full Name
phosphoserine phosphatase
NCBI Official Synonym Full Names
phosphoserine phosphatase
NCBI Official Symbol
PSPH
NCBI Official Synonym Symbols
PSP; PSPHD
NCBI Protein Information
phosphoserine phosphatase
UniProt Protein Name
Phosphoserine phosphatase
Protein Family
UniProt Gene Name
PSPH
UniProt Synonym Gene Names
PSP; PSPase
NCBI Description
The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates.
Research Articles on PSP
Similar Products
Product Notes
The PSP psph (Catalog #AAA200048) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSP antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PSP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the PSP psph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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