Mouse anti-Human Mastermind-Like 3 Monoclonal Antibody | anti-MAMLD1 antibody

Mastermind-Like 3 (MAML3, CAGH3, ERDA3, GDN, TNRC3)

Cat. Num. AAA601554

• Gene Names: MAMLD1; CG1; F18; HYSP2; CXorf6
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Mastermind-Like 3; Monoclonal Antibody; Mastermind-Like 3 (MAML3; CAGH3; ERDA3; GDN; TNRC3); Anti -Mastermind-Like 3 (MAML3; anti-MAMLD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C215
• Specificity: Recognizes human Mastermind-Like 3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-MAMLD1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human Mastermind-Like 3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Related Product Information for anti-MAMLD1 antibody

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1.

Product Categories/Family for anti-MAMLD1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 294489310
• NCBI GeneID: 10046
• NCBI Accession #: NP_001170937.1
• NCBI GenBank Nucleotide #: NM_001177466.2
• UniProt Accession #: Q13495; B2RCQ4; B4DG93; B9EGA5
• Molecular Weight: 83,231 Da
• NCBI Official Full Name: mastermind-like domain-containing protein 1 isoform 3
• NCBI Official Synonym Full Names: mastermind-like domain containing 1
• NCBI Official Symbol: MAMLD1
• NCBI Official Synonym Symbols: CG1; F18; HYSP2; CXorf6
• NCBI Protein Information: mastermind-like domain-containing protein 1
• UniProt Protein Name: Mastermind-like domain-containing protein 1
• Protein Family: Mastermind-like domain-containing protein
• UniProt Gene Name: MAMLD1
• UniProt Synonym Gene Names: CG1; CXorf6
• UniProt Entry Name: MAMD1_HUMAN

NCBI Description

This gene encodes a mastermind-like domain containing protein. This protein may function as a transcriptional co-activator. Mutations in this gene are the cause of X-linked hypospadias type 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

MAMLD1: Transactivates the HES3 promoter independently of NOTCH proteins. HES3 is a non-canonical NOTCH target gene which lacks binding sites for RBPJ. Defects in MAMLD1 are a cause of X-linked hypospadias type 2 (HYSP2). Hypospadias is a common malformation in which the urethra opens on the ventral side of the penis. It is considered a complex disorder with both genetic and environmental factors involved in the pathogenesis. Hypospadias can occur alone on an apparently multifactorial basis or as part of syndromes. Belongs to the mastermind family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; male gonad development

Disease: Hypospadias 2, X-linked