anti-Human, Mouse DAPK3 Antibody | anti-DAPK3 antibody

DAPK3 (Phospho-Thr265) Antibody

Cat. Num. AAA8502496

• Gene Names: DAPK3; DLK; ZIP; ZIPK
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: DAPK3; DAPK3 (Phospho-Thr265) Antibody; DAP kinase 3; DAP-like kinase; Death-associated protein kinase 3; Dlk; EC 2.7.11.1; ZIP-kinase; ZIPK; anti-DAPK3 antibody

• Reactivity: Human, Mouse
• Specificity: DAPK3 (Phospho-Thr265) antibody detects endogenous levels of DAPK3 only when phosphorylated at threonine 265.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 454

• Applicable Applications for anti-DAPK3 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human DAPK3 around the phosphorylation site of threonine 265 (R-M-TP-I-A).
• All Sites: Human: Thr265; Mouse: Thr265
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(P-peptide-+ Western blot analysis of extracts from HuvEc cells, using DAPK3 (Phospho-Thr265) antibody. Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) antibody.)

Product Categories/Family for anti-DAPK3 antibody

Autophagy antibody

References

Kawai T., Mol. Cell. Biol. 18:1642-1651(1998). Murata-Hori M., BS Lett. 451:81-84(1999). Kawai T., Mol. Cell. Biol. 23:6174-6186(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4557511
• NCBI GeneID: 1613
• NCBI Accession #: NP_001339.1
• NCBI GenBank Nucleotide #: NM_001348.2
• UniProt Accession #: O43293; Q05JY4; A0AVN4; B3KQE2
• Molecular Weight: 37,048 Da
• NCBI Official Full Name: death-associated protein kinase 3
• NCBI Official Synonym Full Names: death associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: DLK; ZIP; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK3
• UniProt Synonym Gene Names: ZIPK; DAP kinase 3; Dlk; ZIP-kinase

NCBI Description

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca2+ and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.