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Quality Control (Western blot analysis of immunized recombinant protein, using anti-MARS monoclonal antibody. )

Mouse anti-Human MARS Monoclonal Antibody | anti-MARS antibody

Mouse monoclonal antibody Anti-Human MARS

Gene Names
MARS; MRS; METRS; MTRNS; SPG70
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
Synonyms
MARS; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human MARS; methionine-tRNA synthetase; MTRNS; METRS; anti-MARS antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MARSD10B4
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
2799
Applicable Applications for anti-MARS antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-MARS monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-MARS monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of MARS by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. MARSD10B4-3]Predicted molecular weight: 101 kDa)

Western Blot (WB) (Detection of MARS by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. MARSD10B4-3]Predicted molecular weight: 101 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-MARS mAb. [Lot No. MARSD10B4-2]Predicted molecular weight: 101 kDa)

Immunoprecipitation (IP) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-MARS mAb. [Lot No. MARSD10B4-2]Predicted molecular weight: 101 kDa)

Flow Cytometry (FC/FACS)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-MARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. MARSD10B4-3])

Flow Cytometry (FC/FACS) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-MARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. MARSD10B4-3])

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb. [Lot No. MARSD10B4-2])

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb. [Lot No. MARSD10B4-2])
Related Product Information for anti-MARS antibody
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
61,816 Da
NCBI Official Full Name
Homo sapiens methionyl-tRNA synthetase, mRNA
NCBI Official Synonym Full Names
methionyl-tRNA synthetase
NCBI Official Symbol
MARS
NCBI Official Synonym Symbols
MRS; METRS; MTRNS; SPG70
NCBI Protein Information
methionine--tRNA ligase, cytoplasmic; cytosolic methionyl-tRNA synthetase
UniProt Protein Name
Methionine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
MARS
UniProt Entry Name
SYMC_HUMAN

NCBI Description

This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]

Uniprot Description

MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]

Protein type: EC 6.1.1.10; Other Amino Acids Metabolism - selenoamino acid; Ligase; Translation

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; mitochondrion; cytoplasm; cytosol

Molecular Function: methionine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; methionyl-tRNA aminoacylation; gene expression

Disease: Infantile Liver Failure Syndrome 2; Charcot-marie-tooth Disease, Axonal, Type 2u

Research Articles on MARS

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Product Notes

The MARS mars (Catalog #AAA120060) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human MARS reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARS can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the MARS mars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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