Rabbit anti-Human PHLPP2 Polyclonal Antibody | anti-PHLPP2 antibody

PHLPP2, CT (PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like) (MaxLight 650)

Cat. Num. AAA6333450

• Gene Names: PHLPP2; PHLPPL
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: PHLPP2; Polyclonal Antibody; CT (PHLPP2; KIAA0931; PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like) (MaxLight 650); PH domain leucine-rich repeat-containing protein phosphatase-like; anti-PHLPP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHLPP2
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-PHLPP2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: PHLPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1331 amino acids from the C-terminal region of human PHLPP2.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PHLPP2 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane).PHLPP2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PHLPP2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHLPP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PHLPP2 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PHLPP2 antibody

PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1. This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro-and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation. It also controls the phosphorylation of AKT3.

Product Categories/Family for anti-PHLPP2 antibody

Antibodies; Enzymes; Phosphatase

References

Brognard, J., Mol. Cell 25 (6), 917-931 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 65301141
• NCBI GeneID: 23035
• NCBI Accession #: NP_055835
• NCBI GenBank Nucleotide #: NM_015020.3
• UniProt Accession #: Q6ZVD8; Q9NV17; Q9Y2E3; A1L374
• Molecular Weight: 156 kDa
• NCBI Official Full Name: PH domain leucine-rich repeat-containing protein phosphatase 2 isoform 1
• NCBI Official Synonym Full Names: PH domain and leucine rich repeat protein phosphatase 2
• NCBI Official Symbol: PHLPP2
• NCBI Official Synonym Symbols: PHLPPL
• NCBI Protein Information: PH domain leucine-rich repeat-containing protein phosphatase 2
• UniProt Protein Name: PH domain leucine-rich repeat-containing protein phosphatase 2
• Protein Family: PH domain leucine-rich repeat-containing protein phosphatase
• UniProt Gene Name: PHLPP2
• UniProt Synonym Gene Names: KIAA0931; PHLPPL; PHLPP-like
• UniProt Entry Name: PHLP2_HUMAN

Uniprot Description

PHLPP2: Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Tumor suppressor

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: photoreceptor inner segment; cytoplasm; nucleus; cytosol

Molecular Function: metal ion binding; protein serine/threonine phosphatase activity

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; innate immune response; protein amino acid dephosphorylation