Human Claudin 1 (CLDN1) RTU ELISA Kit | CLDN1 rtu elisa kit

Human Claudin 1 (CLDN1) ELISA Kit

Cat. Num. AAA458403

• Gene Names: CLDN1; CLD1; SEMP1; ILVASC
• Reactivity: Human
• Synonyms: Claudin 1 (CLDN1); Human Claudin 1 (CLDN1) ELISA Kit; CLD1; ILVASC; SEMP1; Senescence-Associated Epithelial Membrane Protein 1; CLDN1 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLDN1. No significant cross-reactivity or interference between CLDN1 and analogues was observed.
• Sequence Length: 211

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLDN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLDN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN1 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CLDN1 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLDN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CLDN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10863887
• NCBI GeneID: 9076
• NCBI Accession #: NP_066924.1
• NCBI GenBank Nucleotide #: NM_021101.4
• UniProt Accession #: O95832
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: claudin-1
• NCBI Official Synonym Full Names: claudin 1
• NCBI Official Symbol: CLDN1
• NCBI Official Synonym Symbols: CLD1; SEMP1; ILVASC
• NCBI Protein Information: claudin-1
• UniProt Protein Name: Claudin-1
• Protein Family: Claudin
• UniProt Gene Name: CLDN1
• UniProt Synonym Gene Names: CLD1; SEMP1

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).