Rabbit anti-Human MED27 Polyclonal Antibody | anti-MED27 antibody

MED27 Antibody

Cat. Num. AAA7000131

• Gene Names: MED27; MED3; CRSP8; CRAP34; CRSP34; TRAP37
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%,Protein G purified
• Synonyms: MED27; Polyclonal Antibody; MED27 Antibody; Mediator of RNA polymerase II transcription subunit 27; Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34; CRSP34; anti-MED27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 275

• Applicable Applications for anti-MED27 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Recombinant human Mediator of RNA polymerase II transcription subunit 27 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9421 / sc-9423 / sc-292610
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: MED27 antibody at 0.5ug/ml+A431 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 36,32,15 kDaObserved band size: 35 kDa)

Related Product Information for anti-MED27 antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

NCBI and Uniprot Product Information

• NCBI GI #: 359751479
• NCBI GeneID: 9442
• NCBI Accession #: NP_001240810.1
• NCBI GenBank Nucleotide #: NM_001253881.1
• UniProt Accession #: Q6P2C8; O95401; Q4F964; Q5VTA4; Q5VTA5; Q9BU57; Q9NYR4; V9GYV9
• Molecular Weight: 14,826 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 27 isoform 2
• NCBI Official Synonym Full Names: mediator complex subunit 27
• NCBI Official Symbol: MED27
• NCBI Official Synonym Symbols: MED3; CRSP8; CRAP34; CRSP34; TRAP37
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 27
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 27
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED27
• UniProt Synonym Gene Names: CRSP complex subunit 8
• UniProt Entry Name: MED27_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex; ubiquitin ligase complex

Molecular Function: protein binding; transcription coactivator activity

Biological Process: gene expression; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; transcription initiation from RNA polymerase II promoter