Testing Data
(Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.)
Mouse M13 bacteriophage (gp3) Monoclonal Antibody | anti-III antibody
M13 bacteriophage (gp3) Biotinylated
Notes: Ship at ambient temperature, freeze upon arrival.
Stability: Products are stable for one year from purchase when stored properly.
Testing Data
(Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.)
Testing Data
(Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.)
Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.
Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
NCBI and Uniprot Product Information
Uniprot Description
Function: Plays essential roles both in the entry of the viral genome into the bacterial host and in the budding process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host coreceptor tolA induces injection of the viral DNA into the host cytoplasm. In the budding process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
Subunit structure: Interacts with G6P; this interaction is required for proper integration of G3P and G6P into the virion. Interacts with G8P
By similarity. Interacts with host tolA
By similarity. Ref.2
Subcellular location: Virion. Host membrane; Single-pass type I membrane protein
Potential. Note: Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
Domain: Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain forms a complex with the C-terminal domain of tolA at later stages of the infection process. N1 is connected to N2 by a flexible glycine-rich linker on the phage. The C-terminal domain is required for release of viral particles from the host bacterial membrane and proper integration of G3P and G6P proteins in the virion.
Post-translational modification: In one of the crystallographic structures Trp-39 is oxidized to 1',2'-dihydro-2'-oxotryptophan (Ref.3).
Sequence similarities: Belongs to the inovirus G3P protein family.
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Product Notes
The III iii (Catalog #AAA395216) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's M13 bacteriophage (gp3) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Antibody specific for the gp3 protein. Antibody can be used for immunohistochemistry, Western Blot (1-5 ug/ml), Flow Cytometry (1 ug/10^6 cells) and ELISA. Optimal Concentration should be evaluated by serial dilutions. Researchers should empirically determine the suitability of the III iii for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M13 bacteriophage (gp3), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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