Testing Data
(Neutralization-Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78.)
Goat anti-Human Lipopolysaccharide-induced CXC Chemokine Polyclonal Antibody
Lipopolysaccharide-induced CXC Chemokine (LIX, CXCL5)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Lipopolysaccharide-induced CXC Chemokine; Polyclonal Antibody; Lipopolysaccharide-induced CXC Chemokine (LIX; CXCL5); Anti -Lipopolysaccharide-induced CXC Chemokine (LIX; anti-Lipopolysaccharide-induced CXC Chemokine antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes human CXCL5. Neutralizes human CXCL5 bioactivity. Based on direct ELISA results, this antibody shows less than 20% cross-reactivity with recombinant, human GCP-2 and less than 5% cross-reactivity with recombinant, mouse KC, recombinant, rat CINC-1, recombinant, human GRO, recombinant, human GRO and recombinant, mouse GCP-2. Additionally, in direct ELISA, this antibody shows no cross-reactivity with other chemokines tested.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 5% trehalose.
Applicable Applications for anti-Lipopolysaccharide-induced CXC Chemokine antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blotting, Neutralization, Immunohistochemistry.
Dilution: Western Blotting: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 5ng/lane under non-reducing and reducing conditions.
Neutralization of Human CXCL5 Bioactivity The exact concentration of antibody required to neutralize rhCXCL5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-human CXCL5 antibody was determined to be approximately 0.2-1ug/ml in the presence of 30ng/ml of rhCXCL5, using BaF/3 cells transfected with rhCXCR-2.
Immunohistochemistry: 15ug/ml Paraffin-embedded tissue sections. To measure the ability of the antibody to neutralize the chemoattractant activity of rhCXCL5 for hCXCR-2 transfected BaF/3cells, Recombinant, Human CXCL5 was incubated with various concentrations of the antibody for 15 minutes at RT in a 96 well microplate. Following this preincubation period, 75ul of the cytokine-antibody solution (containing rhCXCL5 at a final concentration of 0.03g/ml and antibody at the concentrations indicated) was transferred to the lower compartment of a 96 well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 0.2 x10e6 cells/well was added to the top chamber. After incubation for 3 hours at 37 degree C in a 5% CO2
humidified incubator, the chamber was carefully disassembled. The cells that migrate through to the lower chamber were transferred to a 96 well plate. Chemotaxis was measured by Resazurin staining of cells that have migrated through the filter. As shown in figure 2, the ND50 for this lot of antibody is approximately 0.2-1ug/ml.
Dilution: Western Blotting: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 5ng/lane under non-reducing and reducing conditions.
Neutralization of Human CXCL5 Bioactivity The exact concentration of antibody required to neutralize rhCXCL5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-human CXCL5 antibody was determined to be approximately 0.2-1ug/ml in the presence of 30ng/ml of rhCXCL5, using BaF/3 cells transfected with rhCXCR-2.
Immunohistochemistry: 15ug/ml Paraffin-embedded tissue sections. To measure the ability of the antibody to neutralize the chemoattractant activity of rhCXCL5 for hCXCR-2 transfected BaF/3cells, Recombinant, Human CXCL5 was incubated with various concentrations of the antibody for 15 minutes at RT in a 96 well microplate. Following this preincubation period, 75ul of the cytokine-antibody solution (containing rhCXCL5 at a final concentration of 0.03g/ml and antibody at the concentrations indicated) was transferred to the lower compartment of a 96 well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 0.2 x10e6 cells/well was added to the top chamber. After incubation for 3 hours at 37 degree C in a 5% CO2
humidified incubator, the chamber was carefully disassembled. The cells that migrate through to the lower chamber were transferred to a 96 well plate. Chemotaxis was measured by Resazurin staining of cells that have migrated through the filter. As shown in figure 2, the ND50 for this lot of antibody is approximately 0.2-1ug/ml.
Immunogen
E. coli-derived Recombinant, Human CXCL5
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Neutralization-Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78.)
Testing Data
(Neutralization-Chemotaxis Induced by CXCL5/ENA‑78 and Neutralization by Human CXCL5/ENA‑78.)
Immunohistochemistry (IHC)
(Immunohistochemistry-CXCL5/ENA‑78 in Human Breast.)
Immunohistochemistry (IHC)
(Immunohistochemistry-CXCL5/ENA‑78 in Human Breast.)
Testing Data
Testing Data
Product Categories/Family for anti-Lipopolysaccharide-induced CXC Chemokine antibody
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Product Notes
The Lipopolysaccharide-induced CXC Chemokine (Catalog #AAA616514) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Lipopolysaccharide-induced CXC Chemokine (LIX, CXCL5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lipopolysaccharide-induced CXC Chemokine can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blotting, Neutralization, Immunohistochemistry. Dilution: Western Blotting: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human CXCL5. The detection limit for Recombinant, Human CXCL5 is approximately 5ng/lane under non-reducing and reducing conditions. Neutralization of Human CXCL5 Bioactivity The exact concentration of antibody required to neutralize rhCXCL5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-human CXCL5 antibody was determined to be approximately 0.2-1ug/ml in the presence of 30ng/ml of rhCXCL5, using BaF/3 cells transfected with rhCXCR-2. Immunohistochemistry: 15ug/ml Paraffin-embedded tissue sections. To measure the ability of the antibody to neutralize the chemoattractant activity of rhCXCL5 for hCXCR-2 transfected BaF/3cells, Recombinant, Human CXCL5 was incubated with various concentrations of the antibody for 15 minutes at RT in a 96 well microplate. Following this preincubation period, 75ul of the cytokine-antibody solution (containing rhCXCL5 at a final concentration of 0.03g/ml and antibody at the concentrations indicated) was transferred to the lower compartment of a 96 well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size) and 0.2 x10e6 cells/well was added to the top chamber. After incubation for 3 hours at 37 degree C in a 5% CO2 humidified incubator, the chamber was carefully disassembled. The cells that migrate through to the lower chamber were transferred to a 96 well plate. Chemotaxis was measured by Resazurin staining of cells that have migrated through the filter. As shown in figure 2, the ND50 for this lot of antibody is approximately 0.2-1ug/ml. Researchers should empirically determine the suitability of the Lipopolysaccharide-induced CXC Chemokine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipopolysaccharide-induced CXC Chemokine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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