Flow Cytometry (FC/FACS)
(Flow cytometry analysis, intracellular staining, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (FITC conjugate) at a concentration of 50 ug/ml.)
anti-Human, Mouse LRRC32 Monoclonal Antibody | anti-LRRC32 antibody
LRRC32 monoclonal antibody (Plato-1) (FITC conjugate)
Gene Names
LRRC32; GARP; D11S833E
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein G-affinity purified.
Synonyms
LRRC32; Monoclonal Antibody; LRRC32 monoclonal antibody (Plato-1) (FITC conjugate); Leucine-rich repeat-containing protein 32; GARP; Garpin; Glycoprotein A repetitions predominant; anti-LRRC32 antibody
Host
Mouse; Purified from concentrated hybridoma tissue culture supernatant.
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Specificity
Recognizes human and mouse LRRC32. Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa.
Purity/Purification
Protein G-affinity purified.
Form/Format
Liquid. In PBS containing 0.09% sodium azide.
Sequence Length
662
Applicable Applications for anti-LRRC32 antibody
Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa.
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa.
Immunogen
Recombinant human LRRC32 (aa 20-627).
Preparation and Storage
Long term: 4 degree C
Short term: 4 degree C
Stable for at least 1 year after receipt when stored at -20 degree C.
Keep conjugated formats at +4 degree C. Do not freeze. Protect from light.
Short term: 4 degree C
Stable for at least 1 year after receipt when stored at -20 degree C.
Keep conjugated formats at +4 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis, intracellular staining, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (FITC conjugate) at a concentration of 50 ug/ml.)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis, intracellular staining, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (FITC conjugate) at a concentration of 50 ug/ml.)
Related Product Information for anti-LRRC32 antibody
LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells.
Product Categories/Family for anti-LRRC32 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
71,979 Da
NCBI Official Full Name
Leucine-rich repeat-containing protein 32
NCBI Official Synonym Full Names
leucine rich repeat containing 32
NCBI Official Symbol
LRRC32
NCBI Official Synonym Symbols
GARP; D11S833E
NCBI Protein Information
leucine-rich repeat-containing protein 32
UniProt Protein Name
Leucine-rich repeat-containing protein 32
Protein Family
UniProt Gene Name
LRRC32
UniProt Synonym Gene Names
D11S833E; GARP; GARP
UniProt Entry Name
LRC32_HUMAN
NCBI Description
This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]
Uniprot Description
LRRC32:
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.5-q14
Cellular Component: integral to plasma membrane
Research Articles on LRRC32
1. GARP is regulated by miRNAs and controls latent TGF-beta1 production by human regulatory T cells.
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Product Notes
The LRRC32 lrrc32 (Catalog #AAA565112) is an Antibody produced from Mouse; Purified from concentrated hybridoma tissue culture supernatant. and is intended for research purposes only. The product is available for immediate purchase. The LRRC32 monoclonal antibody (Plato-1) (FITC conjugate) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LRRC32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow Cytometry (1:20) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~100kDa by Western blot. Calculated molecular weight of the full-length protein is ~72kDa. Researchers should empirically determine the suitability of the LRRC32 lrrc32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRC32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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