Western Blot (WB)
(Western Blot analysis of COLO 320 cells using EPLIN Monoclonal Antibody at dilution of 1:1000)
Mouse anti-Human LIMA1 Monoclonal Antibody | anti-LIMA1 antibody
LIMA1 Monoclonal Antibody
Gene Names
LIMA1; EPLIN; SREBP3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein G purification
Synonyms
LIMA1; Monoclonal Antibody; LIMA1 Monoclonal Antibody; 1110021C24Rik; Epithelial protein lost in neoplasm; Epithelial protein lost in neoplasm beta; EPLIN; FLJ38853; LIM domain and actin binding 1; LIM domain and actin binding protein 1; LIM domain and actin-binding protein 1; MGC131726; SREBP3; Sterol regulatory element binding protein 3; anti-LIMA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6578
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
760
Applicable Applications for anti-LIMA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
IHC: 1:20-1:200
IF: 1:20-1:200
Immunogen
Fusion protein of EPLIN
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of COLO 320 cells using EPLIN Monoclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of COLO 320 cells using EPLIN Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-LIMA1 antibody
EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.
Product Categories/Family for anti-LIMA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 85kDa
Observed: 90kDa; 110kDa
Observed: 90kDa; 110kDa
NCBI Official Full Name
LIM domain and actin-binding protein 1 isoform 1
NCBI Official Synonym Full Names
LIM domain and actin binding 1
NCBI Official Symbol
LIMA1
NCBI Official Synonym Symbols
EPLIN; SREBP3
NCBI Protein Information
LIM domain and actin-binding protein 1
UniProt Protein Name
LIM domain and actin-binding protein 1
Protein Family
UniProt Gene Name
LIMA1
UniProt Synonym Gene Names
EPLIN; SREBP3
NCBI Description
This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
Uniprot Description
Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
Research Articles on LIMA1
Similar Products
Product Notes
The LIMA1 lima1 (Catalog #AAA2542251) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LIMA1 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIMA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the LIMA1 lima1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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