Rabbit anti-Human GALNT3 Polyclonal Antibody | anti-GALNT3 antibody

GALNT3, ID (GALNT3, Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, Protein-UDP acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3) (MaxLight 490)

Cat. Num. AAA6301132

• Gene Names: GALNT3; HHS; HFTC; GalNAc-T3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GALNT3; Polyclonal Antibody; ID (GALNT3; Polypeptide N-acetylgalactosaminyltransferase 3; Polypeptide GalNAc transferase 3; Protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3) (MaxLight 490); UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3; anti-GALNT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GALNT3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GALNT3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 454-481 in the central region of human GALNT3, UniProt Accession #Q14435.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS648688. GALNT3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skin tissue reacted withMBS648688, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS648688 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MCF-7 cells using MBS648688 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GALNT3 antibody

GALNT3 encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein is highly homologous to other family members, however the enzymes have different substrate specificities.

Product Categories/Family for anti-GALNT3 antibody

Antibodies; Enzymes; Glycosyltransferase

References

1. Joseph, L., et.al., Skeletal Radiol. 39 (1), 63-68 (2010). 2. Masi, L., et.al., J Bone Joint Surg Am 91 (5), 1190-1198 (2009). 3. Chefetz, I., et.al, Biochim. Biophys. Acta 1792 (1), 61-67 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 153266878
• NCBI GeneID: 2591
• NCBI Accession #: NP_004473.2
• NCBI GenBank Nucleotide #: NM_004482.3
• UniProt Accession #: Q14435; Q53TG9; Q7Z476
• Molecular Weight: 22,212 Da
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Symbol: GALNT3
• NCBI Official Synonym Symbols: HHS; HFTC; GalNAc-T3
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Gene Name: GALNT3
• UniProt Synonym Gene Names: GalNAc-T3; pp-GaNTase 3

NCBI Description

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

GALNT3: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Plays a central role in phosphate homeostasis. Mutations cause cause familial tumoral calcinosis, a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Overexpressed in many differentiated carcinomas, suggesting that it may serve as a marker of tumor differentiation. Two alternatively spliced human isoforms have been described.

Protein type: EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: extracellular exosome; Golgi apparatus; Golgi membrane; integral component of membrane; membrane; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; carbohydrate binding; manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: carbohydrate metabolic process; fibroblast growth factor receptor signaling pathway; O-glycan processing; protein O-linked glycosylation via serine; protein O-linked glycosylation via threonine

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial