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Typical Testing Data/Standard Curve (for reference only)

uPA assay kit

Human uPA total antigen ELISA assay

Synonyms
uPA; Human uPA total antigen ELISA assay; Human uPA Total Antigen ELISA; uPA assay kit
Ordering
For Research Use Only!
Procedural Notes
1. Use aseptic technique when opening and dispensing reagents.
2. This kit is designed to work properly as provided and instructed. Additions, deletions, or substitutions to the procedure or reagents are not recommended, as they may be detrimental to the assay.
3. Exercise universal precautions during the performance or handling of this kit or any component contained therein.
Sample Collection and Preparation
Samples of human plasma, serum, urine, cell culture media, or tissue extracts may be applied directly to the plate. The assay measures total uPA in the 0.1-50 ng/ml range. Samples giving uPA levels above 50ng/ml should be diluted in plasma or similar fluid devoid of uPA.
Reagent Preparation
1. Dilute the 50 mL of 10x Wash Buffer concentrate to 1x with 450 mL of DI water prior to use.
2. Prepare 100 mL of TBS Buffer: 0.1 M Tris-HCL, 0.15 M NaCl, pH 7.4
3. Prepare 20 mL of 3% BSA Blocking Buffer: 3% BSA in TBS Buffer
Standard Preparation
Reconstitute the Standard according to the attached dilution table. Do not prepare the Standards until you are ready to apply them to the plate.
Preparation and Storage
1. Store this kit and its components at 4 degree C until use.
2. Reconstituted standards and primary may be stored at -70C for later use. DO NOT
freeze/thaw the
Standards and Primary Antibody more than once.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for uPA assay kit
Introduction: Human uPA total antigen assay is intended for the quantitative determination of total plasminogen activator antigen in biological fluids. Urokinase plasminogen activator is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration (1).

Principle of assay: Human uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-human uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
References
1. Kjøller, L: (2002) Biol. Chem.; 383: 5-19
2. Binnema, D.J., et al.: (1986) Thromb. Res.; 43(5): 569-577
3. Kondera-Anasz, Z., et al.: (2000) Med. Sci. Monit.; 6(3): 616-617
4. Ferrier, C.M., et al.: (1999) British Journal of Cancer; 79(9/10): 1534-1541
5. Singh, I., et al.: (2003) Circulation; 107(6): 869-875
6. Yang, Y.H., et al.: (2001) J. Immunol.; 167(2): 1047-1052

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Product Notes

The uPA (Catalog #AAA480480) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "uPA, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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