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Quality Control (Western blot analysis of immunized recombinant protein, using anti-LHX2 monoclonal antibody. )

Mouse anti-Human LHX2 Monoclonal Antibody | anti-LHX2 antibody

Mouse monoclonal antibody Anti-Human LHX2

Gene Names
LHX2; LH2; hLhx2
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry
Synonyms
LHX2; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human LHX2; LIM homeobox 2; LH2; hLhx2; anti-LHX2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LHX2A12G1
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
1320
Applicable Applications for anti-LHX2 antibody
Dot Blot (DB), Immunocytochemistry (ICC)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-LHX2 monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-LHX2 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LHX2 mAb. [Lot No.LHX2A12G1-2])

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LHX2 mAb. [Lot No.LHX2A12G1-2])
Related Product Information for anti-LHX2 antibody
This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
44,373 Da
NCBI Official Full Name
Homo sapiens LIM homeobox 2, mRNA
NCBI Official Synonym Full Names
LIM homeobox 2
NCBI Official Symbol
LHX2
NCBI Official Synonym Symbols
LH2; hLhx2
NCBI Protein Information
LIM/homeobox protein Lhx2; LIM HOX gene 2; LIM homeobox protein 2; homeobox protein LH-2
UniProt Protein Name
LIM/homeobox protein Lhx2
Protein Family
UniProt Gene Name
LHX2
UniProt Entry Name
LHX2_HUMAN

NCBI Description

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq, Jul 2008]

Uniprot Description

LHX2: Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; axon guidance; dorsal/ventral pattern formation; axon extension; retina development in camera-type eye; olfactory bulb development; positive regulation of transcription, DNA-dependent; neural tube closure; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; telencephalon regionalization

Research Articles on LHX2

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Product Notes

The LHX2 lhx2 (Catalog #AAA120071) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human LHX2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LHX2 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the LHX2 lhx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LHX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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