SDS-Page
Ninein Recombinant Protein | NIN recombinant protein
Recombinant Ninein (NIN)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Ninein; Recombinant Ninein (NIN); GSK3B Interacting Protein; Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein; NIN recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
MDEVEQDQHE ARLKELFDSF DTTGTGSLGQ EELTDLCHML SLEEVAPVLQ QTLLQDNLLG RVHFDQFKEA LILILSRTLS NEEHFQEPDC SLEAQPKYVR GGKRYGRRSL PEFQESVEEFPEVTVIEPLD EEARPSHIPA GDCSEHWKTQ RSE
Sequence Length
2096
Applicable Applications for NIN recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
21.3kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Met1~Glu153
Organism Species
Human
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
Uniprot Description
NIN: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Microtubule-binding; Cytoskeletal
Chromosomal Location of Human Ortholog: 14q22.1
Cellular Component: centriole; centrosome; microtubule; nucleolus; pericentriolar material; spindle pole
Molecular Function: calcium ion binding; GTP binding; protein binding
Biological Process: centrosome localization
Disease: Seckel Syndrome 7
Similar Products
Product Notes
The NIN nin (Catalog #AAA2033098) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ninein can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the NIN nin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDEVEQDQHE ARLKELFDSF DTTGTGSLGQ EELTDLCHML SLEEVAPVLQ QTLLQDNLLG RVHFDQFKEA LILILSRTLS NEEHFQEPDC SLEAQPKYVR GGKRYGRRSL PEFQESVEEF PEVTVIEPLD EEARPSHIPA GDCSEHWKTQ RSE. It is sometimes possible for the material contained within the vial of "Ninein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
