Western Blot (WB)
(RYBP antibody (pAb) tested by Western blot. Nuclear extract of mouse ES cells (30 ug) probed with RYBP antibody (pAb) at a dilution of 1:500.)
Rabbit anti-Human, Mouse RYBP Polyclonal Antibody | anti-RYBP antibody
RYBP antibody (pAb)
Gene Names
RYBP; AAP1; DEDAF; YEAF1; APAP-1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
RYBP; Polyclonal Antibody; RYBP antibody (pAb); AAP1; DEDAF; YEAF1; anti-RYBP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-RYBP antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the C-terminal region of human RYBP.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(RYBP antibody (pAb) tested by Western blot. Nuclear extract of mouse ES cells (30 ug) probed with RYBP antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(RYBP antibody (pAb) tested by Western blot. Nuclear extract of mouse ES cells (30 ug) probed with RYBP antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-RYBP antibody
Short Description: Our RYBP antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: RYBP (RING1 and YY1 Binding Protein) is a member of a non-canonical PRC1 repressive complex composed of at least RING1B and ubiquitinated H2A. Also inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of transcription as a repressor of the transcriptional activity of E4TF1.
Background: RYBP (RING1 and YY1 Binding Protein) is a member of a non-canonical PRC1 repressive complex composed of at least RING1B and ubiquitinated H2A. Also inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of transcription as a repressor of the transcriptional activity of E4TF1.
Product Categories/Family for anti-RYBP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27.4kDa (252aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
RING1 and YY1-binding protein
NCBI Official Synonym Full Names
RING1 and YY1 binding protein
NCBI Official Symbol
RYBP
NCBI Official Synonym Symbols
AAP1; DEDAF; YEAF1; APAP-1
NCBI Protein Information
RING1 and YY1-binding protein
UniProt Protein Name
RING1 and YY1-binding protein
Protein Family
UniProt Gene Name
RYBP
UniProt Synonym Gene Names
DEDAF; YEAF1; APAP-1; DED-associated factor
UniProt Entry Name
RYBP_HUMAN
Uniprot Description
RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 3p13
Cellular Component: nucleoplasm; cytoplasm; PcG protein complex
Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity
Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter
Research Articles on RYBP
Similar Products
Product Notes
The RYBP rybp (Catalog #AAA388907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RYBP antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RYBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the RYBP rybp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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