Mouse LASS3 Monoclonal Antibody | anti-LASS3 antibody

LASS3 (LAG1 Homolog, Ceramide Synthase 3, CerS3, MGC27091) (HRP)

Cat. Num. AAA6183473

• Gene Names: CERS3; ARCI9; LASS3
• Applications: Western Blot
• Purity: Purified
• Synonyms: LASS3; Monoclonal Antibody; LASS3 (LAG1 Homolog; Ceramide Synthase 3; CerS3; MGC27091) (HRP); LAG1 Homolog; MGC27091; anti-LASS3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6C12
• Specificity: Recognizes LASS3.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-LASS3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: LASS3 (NP_849164, 57aa-134aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: EKFVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPSRLKKFQE
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged LASS3 is 0.1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of LASS3 expression in transfected 293T cell line by LASS3 monoclonal antibody (M02), clone 6C12.Lane 1: LASS3 transfected lysate (Predicted MW: 42.13 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB)

(LASS3 monoclonal antibody (M02), clone 6C12. Western Blot analysis of LASS3 expression in MCF-7.)

Western Blot (WB)

(LASS3 monoclonal antibody (M02), clone 6C12. Western Blot analysis of LASS3 expression in Raw 264.7.)

Western Blot (WB)

(LASS3 monoclonal antibody (M02), clone 6C12. Western Blot analysis of LASS3 expression in PC-12.)

Product Categories/Family for anti-LASS3 antibody

Antibodies; Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 116235446
• NCBI GeneID: 204219
• NCBI Accession #: NP_849164
• NCBI GenBank Nucleotide #: NM_178842
• UniProt Accession #: Q8IU89
• Molecular Weight: 46kDa
• NCBI Official Full Name: ceramide synthase 3 isoform 2
• NCBI Official Synonym Full Names: ceramide synthase 3
• NCBI Official Symbol: CERS3
• NCBI Official Synonym Symbols: ARCI9; LASS3
• NCBI Protein Information: ceramide synthase 3
• UniProt Protein Name: Ceramide synthase 3
• UniProt Gene Name: CERS3
• UniProt Synonym Gene Names: LASS3; CerS3
• UniProt Entry Name: CERS3_HUMAN

NCBI Description

This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

Function: May be involved in sphingolipid synthesis or its regulation

By similarity.

Subcellular location: Nucleus membrane; Multi-pass membrane protein

Potential.

Sequence similarities: Contains 1 homeobox DNA-binding domain.Contains 1 TLC (TRAM/LAG1/CLN8) domain.