Flow Cytometry (FC/FACS)
(Separation of HeLa cells stained using anti-human LAR (W7C6) APC antibody (concentration in sample 1,7 ug/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1,7 ug/ml, same as LAR APC concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.)
Mouse LAR Monoclonal Antibody | anti-LAR antibody
Anti-Hu LAR APC
Reactivity
Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
LAR; Monoclonal Antibody; Anti-Hu LAR APC; Leucocyte common antigen related molecule; PTPRF; W7C6; protein tyrosine phosphatase receptor type F; anti-LAR antibody
Host
Mouse
Reactivity
Non-human primates, Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
W7C6
Specificity
The mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for anti-LAR antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: Recommended dilution: 1-4ug/ml
Conjugation
APC
Immunogen
WERI-RB-1 retinoblastoma cells
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Flow Cytometry (FC/FACS)
(Separation of HeLa cells stained using anti-human LAR (W7C6) APC antibody (concentration in sample 1,7 ug/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1,7 ug/ml, same as LAR APC concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.)
Flow Cytometry (FC/FACS)
(Separation of HeLa cells stained using anti-human LAR (W7C6) APC antibody (concentration in sample 1,7 ug/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1,7 ug/ml, same as LAR APC concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.)
Related Product Information for anti-LAR antibody
LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR Functional Application (FUNC)tion.
References
*Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LAR (Catalog #AAA1801870) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu LAR APC reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the LAR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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