Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.)
Mouse anti-Rat, Hamster LAMP1 Monoclonal Antibody | anti-Lamp1 antibody
LAMP1 Antibody
Gene Names
Lamp1; LGP120
Reactivity
Rat, Hamster
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Synonyms
LAMP1; Monoclonal Antibody; LAMP1 Antibody; CD107; CD107a; LAMPA; LGP120; IgpA; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; 120 kDa lysosomal membrane glycoprotein; Lamp-1; anti-Lamp1 antibody
Host
Mouse
Reactivity
Rat, Hamster
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Ly1C6
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
407
Applicable Applications for anti-Lamp1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
1 ug/ml was sufficient for detection by immunoblot analysis
Certificate of Analysis
1ug/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.)
Western Blot (WB)
(Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Related Product Information for anti-Lamp1 antibody
Background Info: Detects an ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.
Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
Product Categories/Family for anti-Lamp1 antibody
References
1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68. 2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76. 4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19. 5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847. 6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.
1. Hiasa, M. et al. (2014). Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets. Physiol Rep. 2(6). pii: e12034. doi: 10.14814/phy2.12034
1. Hiasa, M. et al. (2014). Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets. Physiol Rep. 2(6). pii: e12034. doi: 10.14814/phy2.12034
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,969 Da
NCBI Official Full Name
lysosome-associated membrane glycoprotein 1
NCBI Official Synonym Full Names
lysosomal-associated membrane protein 1
NCBI Official Symbol
Lamp1
NCBI Official Synonym Symbols
LGP120
NCBI Protein Information
lysosome-associated membrane glycoprotein 1; LAMP-1; LGP-120; lysosomal membrane glycoprotein 1; CD107 antigen-like family member A; lysosome-associated membrane protein 1; 120 kDa lysosomal membrane glycoprotein; Lysosomal associated membrane protein 1 (120 kDa)
UniProt Protein Name
Lysosome-associated membrane glycoprotein 1
Protein Family
UniProt Gene Name
Lamp1
UniProt Synonym Gene Names
Lamp-1; LAMP-1; Lysosome-associated membrane protein 1; LGP-120
UniProt Entry Name
LAMP1_RAT
NCBI Description
a lysosomal acidic membrane protein [RGD, Feb 2006]
Uniprot Description
LAMP1: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Belongs to the LAMP family.
Protein type: Membrane protein, integral
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Product Notes
The Lamp1 lamp1 (Catalog #AAA802090) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAMP1 Antibody reacts with Rat, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's LAMP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). 1 ug/ml was sufficient for detection by immunoblot analysis. Researchers should empirically determine the suitability of the Lamp1 lamp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAMP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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