Mouse LAMP1 Monoclonal Antibody
Anti-LAMP1 Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunocytochemistry, Immunoprecipitation
Synonyms
LAMP1; Monoclonal Antibody; Anti-LAMP1 Monoclonal Antibody; LAMP1 Antibody; anti-LAMP1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
LAMP1 Antibody [Mouse monoclonal antibody recognizes LAMP1 of rat and hamster]
This antibody recognizes LAMP1 of rat and hamster
This antibody recognizes LAMP1 of rat and hamster
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-LAMP1 antibody
Immunoblot, Immunocytochemistry, Immunoprecipitation
Application Notes
Immunoblotting: use at 1ug/ml. A band of ~120 kDa is detected.
Immunocytochemistry: use at 5-10ug/ml.Immunoprecipitation: use at 5ug/ml
Affinity purification of LAMP1: use at 2-4mg/ml of CnBR-activated Sepharose 4B.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat liver microsomes
Immunocytochemistry: use at 5-10ug/ml.Immunoprecipitation: use at 5ug/ml
Affinity purification of LAMP1: use at 2-4mg/ml of CnBR-activated Sepharose 4B.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat liver microsomes
Antigen
Rat liver lysosome membranes
Preservatives
0.09% sodium azide
Other Additives
50% glycerol
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-LAMP1 antibody
Lysosome-associated membrane proteins (LAMP1 and LAMP2) are major constituents of the lysosomal membrane. These two proteins have closely related structures with 37% sequence homology. Both are transmembrane glycoproteins localized primarily in lysosomes and late endosomes. Upon cell activation, rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine-based motif (YXXI). Disruption of the spacing between the tyrosine-based motif abolishes lysosome localization of LAMP1, and the altered protein cycles between the membrane and the endosome. Cell surface LAMP1 and LAMP2 promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium which suggests that they are involved in adhesion of PBMC at sites of inflammation.
Product Categories/Family for anti-LAMP1 antibody
References
Lewis, V et al. 1985 J Cell Biol 100: 1839-1847
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Product Notes
The LAMP1 (Catalog #AAA190485) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LAMP1 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunocytochemistry, Immunoprecipitation. Immunoblotting: use at 1ug/ml. A band of ~120 kDa is detected.Immunocytochemistry: use at 5-10ug/ml.Immunoprecipitation: use at 5ug/ml Affinity purification of LAMP1: use at 2-4mg/ml of CnBR-activated Sepharose 4B. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat liver microsomes. Researchers should empirically determine the suitability of the LAMP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAMP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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