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Western Blot (WB) (The anti-LOK Pab is used in Western blot to detect LOK in Jurkat cell lysate.)

Rabbit anti-Human STK10 Polyclonal Antibody | anti-STK10 antibody

STK10, CT (Serine/threonine Kinase 10, Serine/threonine-protein Kinase 10, Lymphocyte-oriented Kinase, LOK, PRO2729) (FITC)

Gene Names
STK10; LOK; PRO2729
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
STK10; Polyclonal Antibody; CT (Serine/threonine Kinase 10; Serine/threonine-protein Kinase 10; Lymphocyte-oriented Kinase; LOK; PRO2729) (FITC); EC=2.7.11.1; anti-STK10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LOK.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-STK10 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human LOK (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-LOK Pab is used in Western blot to detect LOK in Jurkat cell lysate.)

Western Blot (WB) (The anti-LOK Pab is used in Western blot to detect LOK in Jurkat cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-STK10 antibody
References
1. Kuramochi, S., et al., Immunogenetics 49(5):369-375 (1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112,135 Da
NCBI Official Full Name
serine/threonine-protein kinase 10
NCBI Official Synonym Full Names
serine/threonine kinase 10
NCBI Official Symbol
STK10
NCBI Official Synonym Symbols
LOK; PRO2729
NCBI Protein Information
serine/threonine-protein kinase 10; lymphocyte-oriented kinase
UniProt Protein Name
Serine/threonine-protein kinase 10
UniProt Gene Name
STK10
UniProt Synonym Gene Names
LOK
UniProt Entry Name
STK10_HUMAN

NCBI Description

This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

LOK: a protein kinase of the STE20 family. Highly expressed in hematopoietic tissue. May cooperate with SLK to regulate Plk1 function in human cells.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; SLK subfamily

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; polo kinase kinase activity; protein homodimerization activity; ATP binding

Biological Process: protein amino acid autophosphorylation; cell cycle; protein amino acid phosphorylation

Research Articles on STK10

Similar Products

Product Notes

The STK10 stk10 (Catalog #AAA6501158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK10, CT (Serine/threonine Kinase 10, Serine/threonine-protein Kinase 10, Lymphocyte-oriented Kinase, LOK, PRO2729) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK10 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the STK10 stk10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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