Lambda Light Chain Secondary Antibody

Lambda Light Chain (MaxLight 405)

Cat. Num. AAA6188596

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Lambda Light Chain; Monoclonal Secondary Antibody; Lambda Light Chain (MaxLight 405); Lambda Light Chain secondary antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: HP6054
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for Lambda Light Chain secondary antibody: FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FLISA: 1:1000; IHC: 1:10-50; WB: 1:500-16000; Applications are based on unconjugated antibody.
• Immunogen: Lambda light chain Mab is generated from mouse immunized with Lambda light chain recombinant protein.
• Conjugate: MaxLight405
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for Lambda Light Chain secondary antibody

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2).

Product Categories/Family for Lambda Light Chain secondary antibody

Antibodies; IgG Lambda

References

58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 58067: Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

NCBI and Uniprot Product Information

• NCBI GI #: 126541
• NCBI GeneID: 3535
• UniProt Accession #: P01701; P0CG04
• Molecular Weight: ~22.5kDa
• NCBI Official Full Name: Ig lambda chain V-I region NEW
• UniProt Protein Name: Ig lambda chain V-I region NEW
• UniProt Entry Name: LV103_HUMAN

Uniprot Description

IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303|PubMed:20176268}