Rabbit anti-Human MITF Polyclonal Antibody | anti-MITF antibody

MITF, NT (MITF, BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32) (AP)

Cat. Num. AAA6320910

• Gene Names: MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MITF; Polyclonal Antibody; NT (MITF; BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32) (AP); Class E basic helix-loop-helix protein 32; anti-MITF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MITF.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 520

• Applicable Applications for anti-MITF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-28 in the N-terminal region of human MITF, Swiss/UniProt Accession: O75030. Species sequence homology: mouse
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS641933. This demonstrates that MBS641933 detected the MITF protein (arrow).)

Testing Data

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Testing Data

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Related Product Information for anti-MITF antibody

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-MITF antibody

Antibodies; Transcription Factors

References

1. Wang, Y., et al. BMC Med 8, 14 (2010). 2. Shiohara, M., et al. Int J Lab Hematol 31(2):215-226(2009). 3. Hershey, C.L., et al. Gene 347(1):73-82(2005). 4. Miller, A.J., et al. J. Biol. Chem. 280(1):146-155(2005). 5. Shibahara, S., et al. J. Investig. Dermatol. Symp. Proc. 6(1):99-104(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 38156699
• NCBI GeneID: 4286
• NCBI Accession #: NP_937802.1; NP_001171896.1
• NCBI GenBank Nucleotide #: NP_937802.1
• UniProt Accession #: O75030
• NCBI Official Full Name: microphthalmia-associated transcription factor isoform 1
• NCBI Official Synonym Full Names: melanocyte inducing transcription factor
• NCBI Official Symbol: MITF
• NCBI Official Synonym Symbols: MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: MITF
• UniProt Synonym Gene Names: BHLHE32; bHLHe32
• UniProt Entry Name: MITF_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Uniprot Description

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 3p14.2-p14.1

Cellular Component: protein complex; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; cell fate commitment; camera-type eye development; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; melanocyte differentiation; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; regulation of cell proliferation

Disease: Albinism, Ocular, With Sensorineural Deafness; Waardenburg Syndrome, Type 2a; Melanoma, Cutaneous Malignant, Susceptibility To, 8; Tietz Syndrome