Mouse anti-Human Lambda light chain Monoclonal Antibody | anti-LV103 antibody

Lambda light chain Antibody

Cat. Num. AAA9200064

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Synonyms: Lambda light chain; Monoclonal Antibody; Lambda light chain Antibody; Ig lambda chain V-I region NEW; LV103; anti-LV103 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: HP6054
• Specificity: This Lambda light chain monoclonal antibody is generated from mouse immunized with Lambda light chain recombinant protein.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 111

• Applicable Applications for anti-LV103 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:500~16000; IHC~~1:10~50
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Lambda light chain Antibody western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the Lambda light chain antibody detected the Lambda light chain protein (arrow).)

Immunohistochemistry (IHC)

(Lambda light chain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Lambda light chain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LV103 antibody

Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2).

Product Categories/Family for anti-LV103 antibody

Immunology

References

58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201
58067: Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

NCBI and Uniprot Product Information

• NCBI GI #: 126541
• UniProt Accession #: P01701
• Molecular Weight: 11453
• NCBI Official Full Name: Ig lambda chain V-I region NEW, partial
• UniProt Protein Name: Ig lambda chain V-I region NEW
• UniProt Entry Name: LV103_HUMAN

Uniprot Description

IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303|PubMed:20176268}