Mouse anti-Mouse Lactoferrin Monoclonal Antibody | anti-LTF antibody

Lactoferrin, Mouse, mAb 1A10

Cat. Num. AAA584720

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: Lactoferrin; Monoclonal Antibody; Mouse; mAb 1A10; anti-LTF antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1A10
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 475

• Applicable Applications for anti-LTF antibody: Flow Cytometry (FC/FACS), Immuno Assay (IA)
• Application Notes: Flow Cytometry: Antibody 1A10 stains intracellular. For intracellular staining 293 cells were permeabilized and stained with 2 ug/ml 1A10.; The typical starting working dilution is 1:50.
• Immunogen: Mouse lactoferrin peptide
• Preparation and Storage: Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.

Related Product Information for anti-LTF antibody

Monoclonal antibody clone 1A10 recognizes mouse lactoferrin (LF). The expression of small proteins and peptides with microbial activity, also called antimicrobial peptides (AMPs), is considered to be a primitive mechanism of immunity. AMPs are produced by almost all cell types which are commonly exposed to microbes. Lactoferrin is a multifunctional AMP and a major element of innate immunity but also affects adaptive immune functions. LF is a well conserved mammalian non-haem iron-binding 80kD monomeric glycoprotein of the transferrin family. The protein is found in mucosal secretion like saliva, tears, urine, vaginal fluids, semen, urine and very high concentrations in (colostral) milk (1-7mg/ml). Its protective effects range from direct antimicrobial activities against a large panel of microorganisms, including bacteria, viruses, fungi and parasites, to anti-inflammatory and anticancer activities. The main functions of LF resided in specific domains and have predominantly be found in the N-terminal side. One of the main functions is inhibition of microbial growth by sequestration of iron, which is essential for growth. The N-terminal structure of LF is mainly responsible for this function. The N-terminal domain contains also a serine- protease-like activity which is involved in inactivation of host cell invasion by bacteria. Following infection, LF is released from secondary- granules of neutrophils in blood and inflamed tissue and functions as pattern-recognition receptor. Therefore, LF plasma concentration represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover and can be used as an index for neutrophil activation. In the adaptive immune response, LF promotes the maturation of T-cell precursors into competent helper cells and by differentiation of immature B-cells into antigen presenting cells.

NCBI and Uniprot Product Information

• NCBI GI #: 2104522
• NCBI GeneID: 4057
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactoferrin, partial
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; nucleus; protein complex; secretory granule; specific granule

Molecular Function: heparin binding; iron ion binding; protein binding; protein serine/threonine kinase activator activity

Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; cell redox homeostasis; cellular protein metabolic process; humoral immune response; innate immune response in mucosa; iron assimilation by chelation and transport; negative regulation of apoptosis; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; regulation of cytokine production; regulation of tumor necrosis factor production; retinal homeostasis