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Western Blot (WB) (Western BlotPositive WB detected in: Rat kidney tissueAll lanes: PRPS2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 35,36 KDaObserved band size: 35 KDa)

Rabbit anti-Human, Rat PRPS2 Polyclonal Antibody | anti-PRPS2 antibody

PRPS2 Antibody

Gene Names
PRPS2; PRSII
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
PRPS2; Polyclonal Antibody; PRPS2 Antibody; Ribose-phosphate pyrophosphokinase 2; PPRibP; Phosphoribosyl pyrophosphate synthase II; PRS-II; anti-PRPS2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
321
Applicable Applications for anti-PRPS2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Ribose-phosphate pyrophosphokinase 2 protein (11-106AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat kidney tissueAll lanes: PRPS2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 35,36 KDaObserved band size: 35 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat kidney tissueAll lanes: PRPS2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 35,36 KDaObserved band size: 35 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7052317 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7052317 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7052317 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7052317 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7052317 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7052317 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-PRPS2 antibody
Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,054 Da
NCBI Official Full Name
ribose-phosphate pyrophosphokinase 2 isoform 1
NCBI Official Synonym Full Names
phosphoribosyl pyrophosphate synthetase 2
NCBI Official Symbol
PRPS2
NCBI Official Synonym Symbols
PRSII
NCBI Protein Information
ribose-phosphate pyrophosphokinase 2
UniProt Protein Name
Ribose-phosphate pyrophosphokinase 2
UniProt Gene Name
PRPS2
UniProt Synonym Gene Names
PRS-II

NCBI Description

This gene encodes a phosphoribosyl pyrophosphate synthetase that plays a central role in the synthesis of purines and pyrimidines. The encoded protein catalyzes the synthesis of 5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

PRPS2: Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. Belongs to the ribose-phosphate pyrophosphokinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.6.1; Kinase, other; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: Xp22.2

Molecular Function: ATP binding; identical protein binding; protein binding; protein homodimerization activity; ribose phosphate diphosphokinase activity

Biological Process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Research Articles on PRPS2

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Product Notes

The PRPS2 prps2 (Catalog #AAA7052317) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRPS2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRPS2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the PRPS2 prps2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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