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Western Blot (WB) (Western blot analysis of anti-URKL1 Pab in 293 cell line lysate (35ug/lane). URKL1(arrow) was detected using the purified Pab.)

Rabbit anti-Human Uridine-Cytidine Kinase 1-like 1 Polyclonal Antibody | anti-UCK1L antibody

Uridine-Cytidine Kinase 1-like 1, NT (UCK1L, UCK1-LIKE, Uridine-cytidine kinase-like 1, URKL1) (FITC)

Gene Names
UCKL1; UCK1L; URKL1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
Uridine-Cytidine Kinase 1-like 1; Polyclonal Antibody; NT (UCK1L; UCK1-LIKE; Uridine-cytidine kinase-like 1; URKL1) (FITC); anti-UCK1L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human URKL1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
548
Applicable Applications for anti-UCK1L antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human URKL1 (KLH).
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-URKL1 Pab in 293 cell line lysate (35ug/lane). URKL1(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-URKL1 Pab in 293 cell line lysate (35ug/lane). URKL1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-UCK1L antibody
References
1. Kashuba, E., et al., (er) BMC Cell Biol. 3 (1), 23 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61.141
NCBI Official Full Name
uridine-cytidine kinase-like 1 isoform 1
NCBI Official Synonym Full Names
uridine-cytidine kinase 1 like 1
NCBI Official Symbol
UCKL1
NCBI Official Synonym Symbols
UCK1L; URKL1
NCBI Protein Information
uridine-cytidine kinase-like 1
UniProt Protein Name
Uridine-cytidine kinase-like 1
UniProt Gene Name
UCKL1
UniProt Synonym Gene Names
URKL1
UniProt Entry Name
UCKL1_HUMAN

NCBI Description

The protein encoded by this gene is a uridine kinase. Uridine kinases catalyze the phosphorylation of uridine to uridine monophosphate. This protein has been shown to bind to Epstein-Barr nuclear antigen 3 as well as natural killer lytic-associated molecule. Ubiquitination of this protein is enhanced by the presence of natural killer lytic-associated molecule. In addition, protein levels decrease in the presence of natural killer lytic-associated molecule, suggesting that association with natural killer lytic-associated molecule results in ubiquitination and subsequent degradation of this protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

UCKL1: May contribute to UTP accumulation needed for blast transformation and proliferation. Belongs to the uridine kinase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.48; Transferase; Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; uridine kinase activity; ATP binding

Biological Process: viral reproduction; phosphorylation

Research Articles on UCK1L

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Product Notes

The UCK1L uckl1 (Catalog #AAA6504840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Uridine-Cytidine Kinase 1-like 1, NT (UCK1L, UCK1-LIKE, Uridine-cytidine kinase-like 1, URKL1) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Uridine-Cytidine Kinase 1-like 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UCK1L uckl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Uridine-Cytidine Kinase 1-like 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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