Mouse Ku Monoclonal Antibody | anti-XRCC6 antibody

Ku (p70/p80) (Nuclear Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438589

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human, Primates.; Does not react with Mouse, Rat and Chicken
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to PE
• Synonyms: Ku; Monoclonal Antibody; Ku (p70/p80) (Nuclear Marker) Mouse Monoclonal Antibody; Ku (p70): 70kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kDa subunit; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen; 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86Kd subunit; ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); X-ray repair cross-complementing protein 5 (XRCC5); anti-XRCC6 antibody

• Host: Mouse
• Reactivity: Human, Primates.; Does not react with Mouse, Rat and Chicken
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: KU729
• Specificity: Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
• Purity/Purification: Purified Ab conjugated to PE
• Form/Format: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
• Sequence Length: 609

• Applicable Applications for anti-XRCC6 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT)
• Cellular Localization: Nuclear
• Immunogen: Nuclear extract of human HL-60 cells
• Hu-Chromosome Location: 22q13.2 (p70); 2q35 (p80)
• Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells
• Preparation and Storage: Antibody with azide - store at 4 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometry of human Ku (p70/p80) on K562 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ku Monoclonal Antibody (KU729).)

NCBI and Uniprot Product Information

• NCBI GI #: 573014815
• NCBI GeneID: 2547
• NCBI Accession #: NP_001275905.1
• NCBI GenBank Nucleotide #: NM_001288976.1
• UniProt Accession #: P12956; Q6FG89; Q9UCQ2; Q9UCQ3; B1AHC8
• Molecular Weight: 70kDa; 80kDa
• NCBI Official Full Name: X-ray repair cross-complementing protein 6 isoform 1
• NCBI Official Synonym Full Names: X-ray repair cross complementing 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: Kurtoxin
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
• UniProt Entry Name: XRCC6_HUMAN

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: EC 4.2.99.-; Nuclear receptor co-regulator; Helicase; DNA repair, damage; DNA-binding; RNA-binding; EC 3.6.4.-

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytosol; membrane; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleoplasm; nucleus; transcription factor complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; damaged DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; protein binding; protein C-terminus binding

Biological Process: DNA ligation; DNA recombination; double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein heterotetramerization; regulation of smooth muscle cell proliferation; telomere maintenance