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Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant KCNJ3 protein (red) or empty vector (blue) stained using KCNJ3 antibody)
Mouse KCNJ3 Monoclonal Antibody | anti-KCNJ3 antibody
KCNJ3 antibody
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
KCNJ3 antibody was purified by affinity chromatography.
Synonyms
KCNJ3; Monoclonal Antibody; KCNJ3 antibody; Monoclonal KCNJ3; Anti-KCNJ3; GIRK1; KGA; KIR3.1; KCNJ 3; KCNJ-3; anti-KCNJ3 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3E11
Purity/Purification
KCNJ3 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
501
Applicable Applications for anti-KCNJ3 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
FC: 1:100
IHC: 1:150
WB: 1:500-2000
FC: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.
Cross-Reactivity
Human, Monkey, Mouse
Immunogen
KCNJ3 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3 produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant KCNJ3 protein (red) or empty vector (blue) stained using KCNJ3 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant KCNJ3 protein (red) or empty vector (blue) stained using KCNJ3 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant KCNJ3 protein (Right) or empty vector (Left) detected with KCNJ3 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant KCNJ3 protein (Right) or empty vector (Left) detected with KCNJ3 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of KCNJ3 protein in paraffin embedded Carcinoma of Human bladder tissue using KCNJ3 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of KCNJ3 protein in paraffin embedded Carcinoma of Human bladder tissue using KCNJ3 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant KCNJ3 protein using KCNJ3 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant KCNJ3 protein using KCNJ3 antibody)
Related Product Information for anti-KCNJ3 antibody
Mouse monoclonal KCNJ3 antibody
Product Categories/Family for anti-KCNJ3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
KCNJ3, partial
Protein Family
Similar Products
Product Notes
The KCNJ3 (Catalog #AAA834748) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KCNJ3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the KCNJ3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNJ3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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