Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (open histogram) or 0.25 ug of CD93 antibody (filled histogram). Cells in the monocyte gate were used for analysis.)
Mouse CD93 Monoclonal Antibody | anti-CD93 antibody
CD93 antibody
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
CD93 antibody was purified by affinity chromatography.
Synonyms
CD93; Monoclonal Antibody; CD93 antibody; Monoclonal CD93; Anti-CD93; CD-93; C1qRp; CD 93; C1q Receptor; C1qR; anti-CD93 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
R139
Specificity
Human
Purity/Purification
CD93 antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-CD93 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Biological Significance
The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC?s is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors. The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis. CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (open histogram) or 0.25 ug of CD93 antibody (filled histogram). Cells in the monocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control (open histogram) or 0.25 ug of CD93 antibody (filled histogram). Cells in the monocyte gate were used for analysis.)
Related Product Information for anti-CD93 antibody
Mouse monoclonal CD93 antibody
Product Categories/Family for anti-CD93 antibody
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Product Notes
The CD93 (Catalog #AAA838462) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD93 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the CD93 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD93, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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