Mouse anti-Human Jumonji Domain Containing 2A Monoclonal Antibody | anti-KDM4A antibody

Jumonji Domain Containing 2A (JMJD2A, JHDM3A, KIAA0677)

Cat. Num. AAA601302

• Gene Names: KDM4A; JMJD2; JHDM3A; JMJD2A; KIAA0677
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Jumonji Domain Containing 2A; Monoclonal Antibody; Jumonji Domain Containing 2A (JMJD2A; JHDM3A; KIAA0677); Anti -Jumonji Domain Containing 2A (JMJD2A; anti-KDM4A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8C200
• Specificity: Recognizes human Jumonji Domain Containing 2A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-KDM4A antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot..
• Immunogen: Partial sequence of recombinant full-length protein to human Jumonji Domain Containing 2A
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Related Product Information for anti-KDM4A antibody

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Product Categories/Family for anti-KDM4A antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 119627490
• NCBI GeneID: 9682
• UniProt Accession #: O75164; Q5VVB1
• Molecular Weight: 120,662 Da
• NCBI Official Full Name: jumonji domain containing 2A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 4A
• NCBI Official Symbol: KDM4A
• NCBI Official Synonym Symbols: JMJD2; JHDM3A; JMJD2A; KIAA0677
• NCBI Protein Information: lysine-specific demethylase 4A; OTTHUMP00000008810; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; jumonji C domain-containing histone demethylase 3A; jmjC domain-containing histone demethylation protein 3A
• UniProt Protein Name: Lysine-specific demethylase 4A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM4A
• UniProt Synonym Gene Names: JHDM3A; JMJD2; JMJD2A; KIAA0677
• UniProt Entry Name: KDM4A_HUMAN

NCBI Description

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq]

Uniprot Description

JMJD2A: Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. Ubiquitous. Belongs to the JHDM3 histone demethylase family.

Protein type: EC 1.14.11.-; Oxidoreductase; Demethylase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; histone demethylase activity (H3-K36 specific); methylated histone residue binding

Biological Process: cardiac muscle hypertrophy; histone demethylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent