Rabbit anti-Human JMJD2A Polyclonal Antibody | anti-JMJD2A antibody

JMJD2A, Phosphorylated (Y547) (KDM4A, JHDM3A, JMJD2, JMJD2A, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A) (AP)

Cat. Num. AAA6311642

• Gene Names: KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JMJD2A; Polyclonal Antibody; Phosphorylated (Y547) (KDM4A; JHDM3A; JMJD2; KIAA0677; Lysine-specific demethylase 4A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A) (AP); KDM4A; Jumonji domain-containing protein 2A; anti-JMJD2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JMJD2A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-JMJD2A antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic phosphopeptide mapping to a fragment between amino acids 535-565 surrounding Y547 of human JMJD2A, UniProt Accession #O75164
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot Blot analysis of MBS6011803 on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug/ml.)

Related Product Information for anti-JMJD2A antibody

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Product Categories/Family for anti-JMJD2A antibody

Antibodies; Enzymes; Demethylase

References

1. Trojer, P., et al. J. Biol. Chem. 284(13):8395-8405(2009). 2. Lee, J., et al. Nat. Struct. Mol. Biol. 15(1):109-111(2008). 3. Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 55665236
• NCBI GeneID: 9682
• UniProt Accession #: O75164; Q5VVB1
• Molecular Weight: 54,697 Da
• NCBI Official Synonym Full Names: lysine demethylase 4A
• NCBI Official Symbol: KDM4A
• NCBI Official Synonym Symbols: JMJD2; JHDM3A; JMJD2A; TDRD14A
• NCBI Protein Information: lysine-specific demethylase 4A
• UniProt Protein Name: Lysine-specific demethylase 4A
• UniProt Gene Name: KDM4A
• UniProt Synonym Gene Names: JHDM3A; JMJD2; JMJD2A; KIAA0677
• UniProt Entry Name: KDM4A_HUMAN

NCBI Description

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]

Uniprot Description

JMJD2A: Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. Ubiquitous. Belongs to the JHDM3 histone demethylase family.

Protein type: Transcription, coactivator/corepressor; Demethylase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; histone demethylase activity (H3-K36 specific); methylated histone residue binding

Biological Process: cardiac muscle hypertrophy; histone demethylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; negative regulation of transcription, DNA-dependent